Department of Pharmaceutical Sciences, Dibrugarh University, Assam, India.
Environ Toxicol Pharmacol. 2010 Jan;29(1):64-9. doi: 10.1016/j.etap.2009.10.002. Epub 2009 Nov 6.
The present study was undertaken to evaluate the protective effect of an aqueous extract of Corchorus olitorius leaves (AECO) against NaAsO(2) induced brain toxicity in experimental rats. The animals exposed to NaAsO(2) (10mg/kg, p.o.) for 10 days exhibited a significant inhibition (p<0.01) of superoxide dismutase, catalase, glutathione-S-transferase, glutathione peroxidase, glutathione reductase and reduced glutathione levels in rat brain. In addition, the toxin increased (p<0.01) the levels of oxidized glutathione and thiobarbituric acid reactive substances in the brain tissue of experimental rats. Treatment with AECO (50 and 100mg/kg, p.o.) for 15 days prior to arsenic intoxication significantly improved antioxidant markers in a dose dependant manner. Histological studies on the ultrastructural changes of brain tissue supported the protective activity of the AECO. The results suggest that treatment with AECO prior to arsenic intoxication has a significant role in protecting animals from arsenic-induced toxicity.
本研究旨在评估蕹菜叶水提物（AECO）对亚砷酸钠（NaAsO2）诱导的实验大鼠脑毒性的保护作用。暴露于 NaAsO2（10mg/kg，口服）10 天的动物表现出大脑中超氧化物歧化酶、过氧化氢酶、谷胱甘肽-S-转移酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶和还原型谷胱甘肽水平的显著抑制（p<0.01）。此外，该毒素增加了实验大鼠脑组织中氧化型谷胱甘肽和硫代巴比妥酸反应物质的水平。在砷中毒前用 AECO（50 和 100mg/kg，口服）治疗 15 天，可显著改善抗氧化标志物的水平，呈剂量依赖性。对脑组织超微结构变化的组织学研究支持 AECO 的保护作用。结果表明，在砷中毒前用 AECO 治疗对动物具有显著的抗砷毒性作用。
