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白罗斯真杆菌对氧化应激 SH-SY5Y 细胞和动物的神经保护作用。

Neuroprotective effect of Ruminococcus albus on oxidatively stressed SH-SY5Y cells and animals.

机构信息

Department of Public Health Science (Brain Korea 21 PLUS program), Graduate School, Korea University, Seoul, 136-701, Republic of Korea.

School of Biosystem and Biomedical Science, College of Health Science, Korea University, Seoul, 136-701, Republic of Korea.

出版信息

Sci Rep. 2017 Nov 6;7(1):14520. doi: 10.1038/s41598-017-15163-5.

DOI:10.1038/s41598-017-15163-5
PMID:29109537
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5674049/
Abstract

Recent evidence shows that the gut microbiota has an important role in gut-brain crosstalk and is linked to neuronal disorders. The aim of this study was to investigate the effects of intestinal Ruminococcus albus with probiotic potential on neuroprotection in oxidatively stressed SH-SY5Y neuroblastoma cells and animals. To investigate these effects, conditioned medium was prepared using Caco-2 cells cultured with heat-killed R. albus (CRA-CM). Caco-2 cells cultured with heat-killed R. albus showed increased BDNF expression and BDNF protein levels increased in CRA-CM. CRA-CM up-regulated the protein expression levels of SRF, C-fos and CDK2. In addition, CRA-CM protected SH-SY5Y cells from HO-induced cell death. CRA-CM significantly decreased the Bax/Bcl-2 ratio in oxidatively stressed SH-SY5Y cells. Animal experiments showed that oral administration of heat-killed R. albus for 15 days attenuated the oxidative stress induced by sodium arsenate. Treatment with heat-killed R. albus reduced the level of ROS, and the levels of SOD and GSH increased in oxidatively stressed brains. In conclusion, the secretome prepared from Caco-2 cells cultured with heat-killed R. albus might promote neuronal proliferation through the activation of cell proliferation-related proteins, and heat-killed R. albus protects neurons from oxidative damage by reducing ROS levels and increasing SOD and GSH levels.

摘要

最近的证据表明，肠道微生物群在肠脑交流中起着重要作用，并且与神经元紊乱有关。本研究旨在探讨具有益生菌潜力的肠道罗斯氏菌（Ruminococcus albus）对氧化应激 SH-SY5Y 神经母细胞瘤细胞和动物的神经保护作用。为了研究这些作用，使用与热灭活 R. albus 共培养的 Caco-2 细胞制备了条件培养基（CRA-CM）。与热灭活 R. albus 共培养的 Caco-2 细胞显示 BDNF 表达增加，并且 CRA-CM 中的 BDNF 蛋白水平增加。CRA-CM 上调了 SRF、C-fos 和 CDK2 的蛋白表达水平。此外，CRA-CM 可保护 SH-SY5Y 细胞免受 HO 诱导的细胞死亡。CRA-CM 可显著降低氧化应激 SH-SY5Y 细胞中 Bax/Bcl-2 的比值。动物实验表明，口服热灭活 R. albus 15 天可减轻亚砷酸钠诱导的氧化应激。热灭活 R. albus 处理可降低 ROS 水平，并且氧化应激大脑中的 SOD 和 GSH 水平增加。总之，用热灭活 R. albus 共培养的 Caco-2 细胞制备的分泌组可能通过激活与细胞增殖相关的蛋白来促进神经元增殖，并且热灭活 R. albus 通过降低 ROS 水平和增加 SOD 和 GSH 水平来保护神经元免受氧化损伤。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e67/5674049/1fb068619c33/41598_2017_15163_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e67/5674049/4350b238bb55/41598_2017_15163_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e67/5674049/87a6b51ce4a6/41598_2017_15163_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e67/5674049/8506b43d2719/41598_2017_15163_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e67/5674049/9eefc4cacdd5/41598_2017_15163_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e67/5674049/f1f454e59d3a/41598_2017_15163_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e67/5674049/e55f90b80277/41598_2017_15163_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e67/5674049/3411edc7feaa/41598_2017_15163_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e67/5674049/1fb068619c33/41598_2017_15163_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e67/5674049/4350b238bb55/41598_2017_15163_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e67/5674049/87a6b51ce4a6/41598_2017_15163_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e67/5674049/8506b43d2719/41598_2017_15163_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e67/5674049/9eefc4cacdd5/41598_2017_15163_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e67/5674049/f1f454e59d3a/41598_2017_15163_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e67/5674049/e55f90b80277/41598_2017_15163_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e67/5674049/3411edc7feaa/41598_2017_15163_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e67/5674049/1fb068619c33/41598_2017_15163_Fig8_HTML.jpg

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