IRIS - International Research Institute of Stavanger, N-4068 Stavanger, Norway; University of Stavanger, N-4036 Stavanger, Norway.
Environ Toxicol Pharmacol. 2010 Nov;30(3):224-44. doi: 10.1016/j.etap.2010.08.004. Epub 2010 Sep 18.
The determination of polycyclic aromatic hydrocarbon (PAH) metabolites in bile can serve as a tool for assessing environmental PAH exposure in fish. Biliary PAH metabolite levels can be measured using several analytical methods, including simple fluorescence assays (fixed fluorescence detection or synchronous fluorescence spectrometry); high-performance liquid chromatography with fluorescence detection (HPLC-F); gas chromatography-mass spectrometry (GC-MS) after deconjugation, extraction and derivatization of the bile sample, and finally by advanced liquid chromatography-tandem mass spectrometry (LC-MS/MS) and gas chromatography-tandem mass spectrometry (GC-MS/MS) methods. The method alternatives are highly different both with regard to their analytical performance towards different PAH metabolite structures as well as in general technical demands and their suitability for different monitoring strategies. In the present review, the state-of-the-art for these different analytical methods is presented and the advantages and limitations of each approach as well as aspects related to analytical quality control and inter-laboratory comparability of data and availability of certified reference materials are discussed.
胆汁中多环芳烃（PAH）代谢物的测定可作为评估鱼类环境 PAH 暴露的工具。胆汁 PAH 代谢物水平可使用多种分析方法进行测量，包括简单荧光测定法（固定荧光检测或同步荧光光谱法）；高效液相色谱法结合荧光检测（HPLC-F）；胆汁样品的去共轭、提取和衍生化后的气相色谱-质谱法（GC-MS），最后通过高级液相色谱-串联质谱法（LC-MS/MS）和气相色谱-串联质谱法（GC-MS/MS）方法。这些方法选择在不同 PAH 代谢物结构的分析性能以及一般技术要求及其对不同监测策略的适用性方面存在很大差异。在本综述中，介绍了这些不同分析方法的最新技术，并讨论了每种方法的优缺点以及与分析质量控制和数据的实验室间可比性以及可用的认证参考材料相关的方面。
