Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Neurosci Res. 2011 Oct;71(2):188-91. doi: 10.1016/j.neures.2011.06.011. Epub 2011 Jul 21.
Recent technical advances in electrophysiological recording and functional imaging from the brain of living animals have promoted our understandings of the brain function, but these in vivo experiments are still technically demanding and often suffer from spontaneous pulsation, i.e., brain movements caused by respiration and heartbeat. Here we report that thoracotomy suppresses the motion artifact to a practically negligible level. This simple method will be useful in a wide variety of in vivo experiments, such as patch-clamp physiology, and optical imaging of neurons, glial cell, and blood vessels.
最近，活体动物脑电生理记录和功能成像方面的技术进步促进了我们对大脑功能的理解，但这些活体实验仍然具有技术挑战性，并且经常受到自发性脉动（即由呼吸和心跳引起的大脑运动）的影响。在这里，我们报告了开胸术可将运动伪影抑制到几乎可以忽略不计的水平。这种简单的方法将在各种活体实验中非常有用，例如膜片钳生理学和神经元、神经胶质细胞和血管的光学成像。
