The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, 725 N. Wolfe St, WBSB 1001, Baltimore, MD 21205, USA.
J Physiol. 2012 Jul 1;590(13):2955-63. doi: 10.1113/jphysiol.2012.228114. Epub 2012 Apr 16.
Two-photon imaging of fluorescence in brain enables analysis of the structure and dynamic activity of neurons and glial cells in living animals. However, vital functions such as beating of the heart cause pulsations in brain tissue, leading to image distortion and loss of resolution. We find that synchronizing imaging scans to the cardiac cycle reduces motion artifacts, significantly improving the resolution of cellular structures. By interlacing multiple heartbeat triggered imaging scans, it was possible to image large brain volumes with negligible distortion. This approach can be readily incorporated into conventional microscopes to achieve substantial reductions in motion artifacts during two-photon imaging.
双光子荧光成像技术可用于分析活体动物中神经元和神经胶质细胞的结构和动态活动。然而，心跳等重要功能会导致脑组织的脉动，从而导致图像失真和分辨率降低。我们发现，将成像扫描与心脏周期同步可以减少运动伪影，显著提高细胞结构的分辨率。通过交错多个由心跳触发的成像扫描，可以对大脑的大体积进行成像，几乎没有变形。这种方法可以很容易地整合到传统显微镜中，以在双光子成像过程中大幅减少运动伪影。
