The United Graduate School of Agricultural Sciences, Ehime University, Matsuyama 790-8566, Japan.
Fitoterapia. 2011 Oct;82(7):1093-101. doi: 10.1016/j.fitote.2011.07.007. Epub 2011 Jul 20.
The present study investigated the cytotoxicity of enhydrin (1), uvedalin (2) and sonchifolin (3) in cervical cancer cells. We have found that SLs 1-3 in doses in range of 0.22-10 μM inhibited cell proliferation and induced apoptosis in both a dose- and time-dependent fashion. A significant cell death induction was supported by morphological studies. The apoptotic effect is associated with caspase-3/7 activation and NF-кB inhibition. Interestingly, enhydrin possessing two epoxide units was found to be the most cytotoxic compound. Therefore it can be assumed that number of epoxides and existence of α-methylene-γ-lactone moiety are essential for the acceleration of apoptosis.
本研究调查了 Enhydrin（1）、Uvedalin（2）和 Sonchifolin（3）在宫颈癌中的细胞毒性。我们发现 SLs 1-3 在 0.22-10 μM 的剂量范围内以剂量和时间依赖的方式抑制细胞增殖并诱导细胞凋亡。形态学研究支持明显的细胞死亡诱导。细胞凋亡效应与 caspase-3/7 激活和 NF-κB 抑制有关。有趣的是，发现含有两个环氧化物单元的 Enhydrin 是最具细胞毒性的化合物。因此，可以假设环氧化物的数量和α-亚甲基-γ-内酯部分的存在对于加速细胞凋亡是必不可少的。
