Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas-IPN, Prol. Carpio y Plan de Ayala s/n, Colonia Santo Tomás, México, D.F., C.P. 11340, México.
Phytomedicine. 2010 Jul;17(8-9):614-20. doi: 10.1016/j.phymed.2009.12.011. Epub 2010 Jan 25.
In this study, we investigated the cytotoxic effect of perezone, a constituent isolated from the roots of Perezia spp. and of its synthetic isomer isoperezone on the K562 human leukemia cell line. Perezone showed greater cytotoxic effect than isoperezone but both compounds were found to induce cytotoxicity trough a caspase-dependent and a caspase-independent mechanisms; important changes in their light scattering properties, phosphatidylserine translocation and mitochondrial membrane potential disruption were detected by cytometry. The mechanism of death induction of each compound showed interesting concentration-dependent differences. Neither compound induced the apoptosis inducing factor.
在这项研究中，我们研究了从 Perezia spp. 的根部分离出的化合物 perezone 及其合成异构体异 perezone 对 K562 人白血病细胞系的细胞毒性作用。与异 perezone 相比，perezone 表现出更强的细胞毒性作用，但这两种化合物都被发现通过 caspase 依赖性和非依赖性机制诱导细胞毒性；通过细胞术检测到它们的光散射特性、磷脂酰丝氨酸易位和线粒体膜电位破坏的重要变化。每种化合物诱导死亡的机制表现出有趣的浓度依赖性差异。这两种化合物都没有诱导凋亡诱导因子。
