Division of Environmental Chemistry, National Institute of Health Sciences, Kamiyoga 1-18-1, Setagaya-ku, Tokyo, Japan.
Toxicol Lett. 2011 Oct 10;206(2):172-7. doi: 10.1016/j.toxlet.2011.07.010. Epub 2011 Jul 20.
We examined the biodistribution of C(60) in rats after tail vein administration using LC-MS/MS. C(60) was detected in various tissues, such as brain, kidneys, liver, lungs, and spleen of rats. On the other hand, no C(60) was found in blood. The highest C(60) concentration was observed in the lungs, followed by spleen, liver, kidneys, and brain. These results suggested that C(60) injected in the tail vein could be filtered by lung capillary vessels and accumulate in the lungs prior to being distributed to other tissues. Moreover, C(60) not being detected in the blood indicates that clearance of C(60) from the blood by filtration might effectively occur in the lungs. The time-dependent variation in the biodistribution of C(60) was evaluated. A time-dependent decrease in C(60) concentrations was observed in all tissues, except spleen. Moreover, a decreasing trend of C(60) levels differed among tissues, which could be due to differences in accumulation. These results suggest that unmodified C(60) and/or C(60) metabolites by metabolic enzymes could be excreted into feces and/or urine. In further studies, the metabolic and excretion pathways of C(60) should be evaluated to understand the toxicokinetics of C(60).
我们使用 LC-MS/MS 研究了 C（60）经尾静脉给药后在大鼠体内的分布。C（60）在大鼠的各种组织中均有检测到，如脑、肾、肝、肺和脾。另一方面，在血液中未发现 C（60）。在肺中观察到的 C（60）浓度最高，其次是脾、肝、肾和脑。这些结果表明，静脉注射到尾静脉的 C（60）可以被肺毛细血管过滤，并在分布到其他组织之前积聚在肺部。此外，血液中未检测到 C（60）表明 C（60）可能通过肺部过滤有效地从血液中清除。评估了 C（60）在体内的时间依赖性变化。除脾外，所有组织中 C（60）浓度均呈时间依赖性下降。此外，组织间 C（60）水平的下降趋势不同，这可能是由于积累的差异所致。这些结果表明，未修饰的 C（60）和/或代谢酶代谢的 C（60）代谢物可能被排泄到粪便和/或尿液中。在进一步的研究中，应评估 C（60）的代谢和排泄途径，以了解 C（60）的毒代动力学。
