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羟基化富勒烯对三种肝细胞的细胞毒性作用。

Cytotoxic Effects of Hydroxylated Fullerenes in Three Types of Liver Cells.

作者信息

Shimizu Kumiko, Kubota Reiji, Kobayashi Norihiro, Tahara Maiko, Sugimoto Naoki, Nishimura Tetsuji, Ikarashi Yoshiaki

机构信息

Division of Environmental Chemistry, National Institute of Health Sciences, Kamiyoga 1-18-1, Setagaya-ku, Tokyo 158-8501, Japan.

Division of Food Additives, National Institute of Health Sciences, Kamiyoga 1-18-1, Setagaya-ku, Tokyo 158-8501, Japan.

出版信息

Materials (Basel). 2013 Jul 9;6(7):2713-2722. doi: 10.3390/ma6072713.

DOI:10.3390/ma6072713
PMID:28811404
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5521227/
Abstract

Fullerenes C have attracted considerable attention in the biomedical field due to their interesting properties. Although there has been a concern that C could be metabolized to hydroxylated fullerenes (C(OH)) , there is little information on the effect of hydroxylated C on liver cells. In the present study, we evaluated the cytotoxic effects of fullerene C and various hydroxylated C derivatives, C(OH)₂, C(OH), C(OH) and C(OH), with three different types of liver cells, dRLh-84, HepG2 and primary cultured rat hepatocytes. C, C(OH)₂ and C(OH) exhibited little or no cytotoxicity in all of the cell types, while C(OH) and C(OH) induced cytotoxic effects in dRLh-84 cells, accompanied by the appearance of numerous vacuoles around the nucleus. Moreover, mitochondrial activity in liver cells was significantly inhibited by C(OH) and C(OH). These results indicate that the number of hydroxyl groups on C(OH) contribute to the difference of their cytotoxic potential and mitochondrial damage in liver cells.

摘要

富勒烯C因其有趣的特性在生物医学领域引起了相当大的关注。尽管有人担心C可能会代谢为羟基化富勒烯（C(OH)），但关于羟基化C对肝细胞的影响却知之甚少。在本研究中，我们用三种不同类型的肝细胞dRLh-84、HepG2和原代培养的大鼠肝细胞评估了富勒烯C和各种羟基化C衍生物C(OH)₂、C(OH)、C(OH)和C(OH)的细胞毒性作用。C、C(OH)₂和C(OH)在所有细胞类型中几乎没有或没有细胞毒性，而C(OH)和C(OH)在dRLh-84细胞中诱导细胞毒性作用，伴随着细胞核周围出现大量空泡。此外，C(OH)和C(OH)显著抑制了肝细胞中的线粒体活性。这些结果表明，C(OH)上的羟基数量导致了它们在肝细胞中的细胞毒性潜力和线粒体损伤的差异。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/897a/5521227/16c666285b46/materials-06-02713-g005a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/897a/5521227/40a8d74ba7ee/materials-06-02713-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/897a/5521227/9c943c7dfa91/materials-06-02713-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/897a/5521227/25eb8fd043d4/materials-06-02713-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/897a/5521227/b6ad990b0f6c/materials-06-02713-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/897a/5521227/16c666285b46/materials-06-02713-g005a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/897a/5521227/40a8d74ba7ee/materials-06-02713-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/897a/5521227/9c943c7dfa91/materials-06-02713-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/897a/5521227/25eb8fd043d4/materials-06-02713-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/897a/5521227/b6ad990b0f6c/materials-06-02713-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/897a/5521227/16c666285b46/materials-06-02713-g005a.jpg

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Time-dependent variation in the biodistribution of C₆₀ in rats determined by liquid chromatography-tandem mass spectrometry.采用液相色谱-串联质谱法研究 C₆₀ 在大鼠体内的生物分布随时间的变化。
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Highly hydroxylated fullerene localizes at the cytoskeleton and inhibits oxidative stress in adipocytes and a subcutaneous adipose-tissue equivalent.
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