Lulla Rishi R, Costa Fabricio F, Bischof Jared M, Chou Pauline M, de F Bonaldo Maria, Vanin Elio F, Soares Marcelo B
Division of Hematology, Oncology and Stem Cell Transplantation, Children's Memorial Hospital, 2300 Children's Plaza, Chicago, IL 60614, USA.
Sarcoma. 2011;2011:732690. doi: 10.1155/2011/732690. Epub 2011 Jul 18.
A limited number of reports have investigated the role of microRNAs in osteosarcoma. In this study, we performed miRNA expression profiling of osteosarcoma cell lines, tumor samples, and normal human osteoblasts. Twenty-two differentially expressed microRNAs were identified using high throughput real-time PCR analysis, and 4 (miR-135b, miR-150, miR-542-5p, and miR-652) were confirmed and validated in a different group of tumors. Both miR-135b and miR-150 have been previously shown to be important in cancer. We hypothesize that dysregulation of differentially expressed microRNAs may contribute to tumorigenesis. They might also represent molecular biomarkers or targets for drug development in osteosarcoma.
仅有少数报告研究了微小RNA在骨肉瘤中的作用。在本研究中，我们对骨肉瘤细胞系、肿瘤样本和正常人成骨细胞进行了微小RNA表达谱分析。使用高通量实时PCR分析鉴定出22种差异表达的微小RNA，其中4种（miR-135b、miR-150、miR-542-5p和miR-652）在另一组肿瘤中得到了确认和验证。miR-135b和miR-150此前均已被证明在癌症中具有重要作用。我们推测，差异表达的微小RNA失调可能有助于肿瘤发生。它们也可能代表骨肉瘤的分子生物标志物或药物开发靶点。
