Bioinformatics Laboratory and National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
PLoS One. 2011;6(7):e21652. doi: 10.1371/journal.pone.0021652. Epub 2011 Jul 18.
The involvement of noncoding RNAs (ncRNAs) in the development of the human brain remains largely unknown. Applying a cloning strategy for detection of intermediate size (50-500 nt) ncRNAs (is-ncRNAs) we have identified 82 novel transcripts in human fetal brain tissue. Most of the novel is-ncRNAs are not well conserved in vertebrates, and several transcripts were only found in primates. Northern blot and microarray analysis indicated considerable variation in expression across human fetal brain development stages and fetal tissues for both novel and known is-ncRNAs. Expression of several of the novel is-ncRNAs was conspicuously absent in one or two brain cancer cell lines, and transient overexpression of some transcripts in cancer cells significantly inhibited cell proliferation. Overall, our results suggest that is-ncRNAs play important roles in the development and tumorigenesis of human brain.
非编码 RNA（ncRNAs）在人类大脑发育中的作用在很大程度上尚不清楚。我们应用一种用于检测中大小（50-500nt）ncRNAs（is-ncRNAs）的克隆策略，在人胎儿脑组织中鉴定出了 82 个新的转录本。大多数新的 is-ncRNAs 在脊椎动物中没有很好的保守性，并且有几个转录本仅在灵长类动物中发现。Northern blot 和微阵列分析表明，新型和已知的 is-ncRNAs 在人类胎儿大脑发育阶段和胎儿组织中的表达存在相当大的差异。几种新型 is-ncRNAs 的表达在一个或两个脑癌细胞系中明显缺失，并且一些转录本在癌细胞中的瞬时过表达显著抑制了细胞增殖。总的来说，我们的研究结果表明，is-ncRNAs 在人类大脑的发育和肿瘤发生中发挥重要作用。
