National Bureau of Fish Genetic Resources, Lucknow, 226002, Uttra Pradesh, India.
Mol Biol Rep. 2012 Apr;39(4):4275-82. doi: 10.1007/s11033-011-1214-3. Epub 2011 Jul 26.
Samples of seven Sisorid catfish species of the genus Glyptothorax (G. garhwali, G. dakpathari, G. brevipinnis, G. ngapang, G. granulus, G. ventrolineatus, and G. davissinghi) were collected from the Himalayan region and the Western Ghats of India. They were analyzed for the mitochondrial cytochrome b gene (Cyt b). Out of 1152 nucleotide positions analyzed, 269 (23.3%) were found to be variable and 235 (20.3%) were parsimoniously informative. The sequences showed 111 (9.6%) fourfold degenerate sites. The overall transition/transversion bias was R = 3.457. The average proportion of base substitutions measured as P-distance for all sequences of seven Glyptothorax species and other five species comprising four comparison groups was intraspecies, P = 0.17 ± 0.05%, intragenus, P = 10.75 ± 0.48%, intrafamily, P = 20.07 ± 1.43%, and intraorder, P = 21.10 ± 0.45%. Within the Glyptothorax genus maximum divergence was obtained among G. brevipinnis sequences, whereas the least divergence was obtained within G. davissinghi. The phylogenetic trees for 193 and 47 sequences of Sisorid catfishes together were developed using the Cyt b gene and four different analytical approaches: Bayesian (BA), neighbour-joining (NJ), maximum parsimony (MP), and maximum likelihood (ML). The analysis revealed a monophyletic origin for the all investigated representatives of the genus Glyptothorax (99, 100, 99, and 73% support level in our BA, NJ, MP, and ML analyses respectively) and with some reservations for Sisoridae, which is the principal family investigated. The monophyletic origin of the two subfamilies of Sisorid catfish defined in the literature was partly also supported by molecular phylogenetic data.
从喜马拉雅山脉地区和印度西高止山脉采集了 7 种纹胸鮡属（Glyptothorax）的长须鲶鱼样本，包括 G. garhwali、G. dakpathari、G. brevipinnis、G. ngapang、G. granulus、G. ventrolineatus 和 G. davissinghi。对这些样本的线粒体细胞色素 b 基因（Cyt b）进行了分析。在分析的 1152 个核苷酸位置中，有 269 个（23.3%）是可变的，有 235 个（20.3%）是简约信息丰富的。这些序列显示出 111 个（9.6%）四重简并位点。总的转换/颠换偏倚为 R = 3.457。对于 7 种纹胸鮡属物种以及其他 5 种包括 4 个比较组的所有序列的平均碱基替换比例测量为 P-距离，所有种内的比例为 P = 0.17 ± 0.05%，属内的比例为 P = 10.75 ± 0.48%，科内的比例为 P = 20.07 ± 1.43%，目内的比例为 P = 21.10 ± 0.45%。在纹胸鮡属内，G. brevipinnis 序列之间的最大分歧，而 G. davissinghi 序列之间的分歧最小。使用 Cyt b 基因和四种不同的分析方法：贝叶斯分析（BA）、邻接法（NJ）、最大简约法（MP）和最大似然法（ML），构建了 193 个和 47 个长须鲶鱼序列的系统发育树。分析结果显示，所有调查的纹胸鮡属代表种都具有单系起源（我们的 BA、NJ、MP 和 ML 分析中的支持率分别为 99%、100%、99%和 73%），并且对于主要研究的 Sisoridae 科也有一些保留意见。文献中定义的两个纹胸鮡亚科的单系起源也部分得到了分子系统发育数据的支持。
