Wang Changsong, Lan Daoliang, Hua Xiuguo
School of Agriculture and Biology, Shanghai JiaoTong University, Shanghai, China.
Virus Genes. 2011 Dec;43(3):350-2. doi: 10.1007/s11262-011-0643-3. Epub 2011 Jul 26.
In this study, a total of 116 stool specimens were collected from pigs of different ages in three pig farms of Shanghai in China during 2010. Forty-five (38.8%) stool specimens were positive for porcine kobuvirus using reverse transcriptase-polymerase chain reaction (RT-PCR). The prevalence rate of porcine kobuvirus in the three pig farms which are located in Minhang District, Qingpu District and Fengxian District was 46.7%(21/45), 35.1%(13/37), and 32.4%(11/34), respectively. We demonstrated that porcine kobuvirus infections are existent in certain domestic pigs in Shanghai and high prevalence of this virus was found in the piglets under the age of 6 weeks and in pigs with diarrhea in Shanghai. Twenty-seven representative strains of porcine kobuvirus detected in this study were randomly selected based on the equal distribution of the sampling date throughout the study and analyzed for their phylogenetic relationships with other kobuvirus reference strains. The phylogenetic analysis suggested the presence of multiple porcine kobuvirus lineages in Shanghai, China.
2010年期间，在中国上海的三个养猪场采集了来自不同年龄段猪的共计116份粪便样本。使用逆转录聚合酶链反应（RT-PCR）检测发现，45份（38.8%）粪便样本猪杯状病毒呈阳性。位于闵行区、青浦区和奉贤区的三个养猪场中，猪杯状病毒的流行率分别为46.7%（21/45）、35.1%（13/37）和32.4%（11/34）。我们证明，上海部分家猪存在猪杯状病毒感染，且在6周龄以下的仔猪和上海腹泻猪中发现该病毒的高流行率。根据整个研究过程中采样日期的均匀分布，从本研究中检测到的27株猪杯状病毒代表性毒株中随机选择，并分析它们与其他杯状病毒参考毒株的系统发育关系。系统发育分析表明，中国上海存在多个猪杯状病毒谱系。
