Yamashita T, Kobayashi S, Sakae K, Nakata S, Chiba S, Ishihara Y, Isomura S
Department of Virology, Aichi Prefectural Institute of Public Health, Nagoya, Japan.
J Infect Dis. 1991 Nov;164(5):954-7. doi: 10.1093/infdis/164.5.954.
Fecal extracts from 12 subjects in outbreaks of oyster-associated nonbacterial gastroenteritis were inoculated with BS-C-1 cells for isolation of the causative viruses. Cytopathic agents were isolated from 3 patients. No cross-neutralizing reactions were observed between the isolates and prototypes of human enteroviruses. The isolates were approximately 30 nm in diameter and had a distinct ultrastructure resembling that of astroviruses. Four polypeptide bands with molecular sizes of 42, 28, 27, and 22 kDa were seen on SDS-PAGE analyses. Seroconversion against the isolate was observed in 18 (31.6%) of 57 patients involved in five of seven outbreaks examined by neutralization test. A protein band characteristically reactive with the paired serum samples was detectable at 42 kDa by immunoblot assay. These results suggested that some small round viruses resembling astroviruses might show cytopathic effect in BS-C-1 cells and may be associated with an oyster-related gastroenteritis.
从12名与牡蛎相关的非细菌性肠胃炎暴发患者的粪便提取物接种到BS-C-1细胞中，以分离致病病毒。从3名患者中分离出了细胞病变因子。在这些分离株与人肠道病毒原型之间未观察到交叉中和反应。这些分离株直径约为30纳米，具有与星状病毒相似的独特超微结构。在SDS-PAGE分析中可见四条分子大小分别为42、28、27和22 kDa的多肽带。通过中和试验检测，在7次暴发中的5次所涉及的57名患者中，有18名（31.6%）出现了针对该分离株的血清转化。通过免疫印迹分析，在42 kDa处可检测到一条与配对血清样本具有特征性反应的蛋白带。这些结果表明，一些类似星状病毒的小圆病毒可能在BS-C-1细胞中表现出细胞病变效应，并且可能与牡蛎相关的肠胃炎有关。
