Verma Harsha, Mor Sunil K, Abdel-Glil Mostafa Y, Goyal Sagar M
Veterinary Diagnostic Laboratory, Department of Veterinary Population Medicine, University of Minnesota, St. Paul, MN 55108, USA.
Virus Genes. 2013 Jun;46(3):551-3. doi: 10.1007/s11262-013-0879-1. Epub 2013 Jan 20.
Porcine kobuvirus has been associated with piglet diarrhea in Asia and Europe, but there are no reports of its presence in the U.S. swine farms. We screened intestinal contents from 114 diarrheic pigs and fecal samples from 46 apparently healthy pigs to determine the presence of kobuvirus by reverse transcription-polymerase chain reaction using 3D (RNA polymerase) region primers (amplicon size 216 bp). The samples from ill pigs came from 15 different U.S. states, while those from healthy pigs were obtained from three different farms in Minnesota. Twenty-five (21.9 %) pigs with diarrhea and ten (21.7 %) healthy pigs were positive for kobuvirus. All strains from diarrheic pigs were further typed by means of VP1 region primers (amplicon size 811 bp). Phylogenetic analysis revealed that all porcine kobuvirus strains had 93.1-96.5 % nucleotide identity with NLD45 strain from the Netherlands and BRA24 strain from Brazil in the 3D region. In the VP1 region, only 86.7-88.5 % homology was found with the T247 strain from Japan and 85.8-87.4 % homology with WUH1 strain from China. All 25 kobuvirus positive pigs had mixed infection with transmissible gastroenteritis virus and/or rotavirus (groups A, B, or C). Pigs less than 4 weeks of age showed higher prevalence of kobuvirus than the older pigs. The results of this preliminary study indicate that porcine kobuvirus is present in both healthy and diarrheic pigs in the U.S. and that further studies are needed to determine its role in gastrointestinal infections of pigs.
猪杯状病毒在亚洲和欧洲与仔猪腹泻有关，但在美国养猪场尚无其存在的报道。我们使用3D（RNA聚合酶）区域引物（扩增片段大小216 bp），通过逆转录-聚合酶链反应筛选了114头腹泻猪的肠道内容物和46头看似健康猪的粪便样本，以确定杯状病毒的存在。患病猪的样本来自美国15个不同的州，而健康猪的样本来自明尼苏达州的三个不同农场。25头（21.9%）腹泻猪和10头（21.7%）健康猪杯状病毒呈阳性。所有腹泻猪的毒株都通过VP1区域引物（扩增片段大小811 bp）进一步分型。系统发育分析显示，所有猪杯状病毒毒株在3D区域与来自荷兰的NLD45毒株和来自巴西的BRA24毒株具有93.1-96.5%的核苷酸同一性。在VP1区域，与来自日本的T247毒株仅发现86.7-88.5%的同源性，与来自中国的WUH1毒株发现85.8-87.4%的同源性。所有25头杯状病毒阳性猪都与传染性胃肠炎病毒和/或轮状病毒（A、B或C组）混合感染。小于4周龄的猪杯状病毒患病率高于年龄较大的猪。这项初步研究的结果表明，猪杯状病毒在美国健康猪和腹泻猪中均存在，需要进一步研究以确定其在猪胃肠道感染中的作用。
