Aino Health Science Center, Aino University, Tokyo, Japan.
Infect Genet Evol. 2010 Oct;10(7):950-4. doi: 10.1016/j.meegid.2010.06.001. Epub 2010 Jun 12.
This study reports the detection and genetic characterization of porcine kobuvirus, a new member of the genus Kobuvirus in the family Picornaviridae. Among 293 fecal specimens collected from healthy pigs in 2009, in Japan, 133 (45.4%) were positive for kobuvirus using RT-PCR screening method. Of the positive specimens, 124 were obtained from pigs < or =6 months old, while 9 samples were from pigs >6 months old. Fifty-two representative strains of kobuviruses detected in this study were randomly selected and analyzed for their phylogenetic relationships with those other kobuvirus reference strains. The phylogenetic tree confirmed that 51 strains belonged to porcine kobuvirus and formed the exclusive branch with other porcine kobuvirus reference strains. In addition, the nucleotide sequence of H023/2009/JP shared very low levels of sequence identity with those of other porcine kobuvirus strains, but showed the highest level of sequence identity with bovine kobuvirus U-1 prototype strain. Our study demonstrated clearly that, porcine kobuvirus infection was common in healthy pigs and high prevalence of this virus was found in younger age of <6 months old of porcine populations in Tokyo and Hokkaido, Japan.
本研究报告了猪博卡病毒的检测和遗传特征，猪博卡病毒是小 RNA 病毒科博卡病毒属的新成员。在 2009 年从日本健康猪采集的 293 份粪便标本中，使用 RT-PCR 筛选方法，有 133 份（45.4%）为博卡病毒阳性。在阳性标本中，有 124 份来自 <或=6 月龄的猪，9 份来自 >6 月龄的猪。从本研究中检测到的 52 株代表性博卡病毒株被随机选择并分析其与其他博卡病毒参考株的系统进化关系。系统进化树证实，51 株属于猪博卡病毒，与其他猪博卡病毒参考株形成独特的分支。此外，H023/2009/JP 的核苷酸序列与其他猪博卡病毒株的序列同一性非常低，但与牛博卡病毒 U-1 原型株的序列同一性最高。我们的研究清楚地表明，猪博卡病毒感染在健康猪中很常见，在日本东京和北海道的 <6 月龄的幼猪群体中，该病毒的高流行率。
