Onda H, Ohkubo S, Ogi K, Kosaka T, Kimura C, Matsumoto H, Suzuki N, Fujino M
Tsukuba Research Laboratories, Takeda Chemical Industries Ltd., Ibaraki, Japan.
FEBS Lett. 1990 Feb 26;261(2):327-30. doi: 10.1016/0014-5793(90)80583-5.
A cDNA encoding human endothelin 3 (ET-3) precursor was cloned from a cDNA library from the placenta, and its nucleotide and deduced amino acid sequences were determined. This ET-3 cDNA was found to contain 2.3 kb pairs and encode prepro-ET-3 protein consisting of 224 amino acid residues. The putative big-ET-3 seems to consist of 42 amino acid residues. Two of the intron insertion sites were determined with information from nucleotide sequences of the cloned genomic ET-3 gene. This is the first direct evidence that the ET-3 gene is transcribed and expressed in the placenta.
从胎盘的cDNA文库中克隆出编码人内皮素3（ET-3）前体的cDNA，并测定了其核苷酸序列和推导的氨基酸序列。发现该ET-3 cDNA含有2.3千碱基对，编码由224个氨基酸残基组成的前内皮素原-3蛋白。推测的大内皮素-3似乎由42个氨基酸残基组成。利用克隆的基因组ET-3基因的核苷酸序列信息确定了两个内含子插入位点。这是ET-3基因在胎盘中转录和表达的首个直接证据。
