Department of Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China.
J Environ Sci (China). 2011;23(5):816-23. doi: 10.1016/s1001-0742(10)60458-5.
Three strains of Gram-negative bacteria capable of removing geosmin from drinking water were isolated from biologically active carbon and identified to be Chryseobacterium sp., Sinorhizobium sp. and Stenotrophomonas sp. based on physio-biochemistry analysis and 16S rRNA gene sequence analysis. Removal efficiencies of 2 mg/L geosmin in mineral salts medium were 84.0%, 80.2% and 74.4% for Chryseobacterium sp., Sinorhizobium sp. and Stenotrophomonas sp., respectively, while removal efficiencies of 560 ng/L geosmin in filter influent were 84.8%, 82.3% and 82.5%, respectively. The biodegradation of geosmin was determined to be a pseudo first-order reaction, with rate constants at 2 mg/L and 560 ng/L being 0.097 and 0.086 day(-1), 0.089 and 0.084 day(-1), 0.074 and 0.098 day(-1) for the above mentioned degraders, respectively. The biomass of culture in the presence of geosmin was much higher than that in the absence of geosmin.
从生物活性炭中分离到三株能去除饮用水中土臭素的革兰氏阴性细菌,根据生理生化分析和 16S rRNA 基因序列分析,鉴定为黄杆菌属、根瘤菌属和寡养单胞菌属。在无机盐培养基中,黄杆菌属、根瘤菌属和寡养单胞菌属对 2mg/L 土臭素的去除率分别为 84.0%、80.2%和 74.4%,而在滤过进水时,对 560ng/L 土臭素的去除率分别为 84.8%、82.3%和 82.5%。土臭素的生物降解被确定为拟一级反应,在 2mg/L 和 560ng/L 时的速率常数分别为 0.097 和 0.086 天(-1)、0.089 和 0.084 天(-1)、0.074 和 0.098 天(-1),分别为上述降解菌。在土臭素存在的情况下,培养物的生物量远高于不存在土臭素的情况。