Ceken Nihan, Yurtsever Sureyya Gul, Baran Nurten, Alper Emrah, Buyrac Zafer, Unsal Belkis
Microbiology and Clinical Microbiology Department, Izmir Ataturk Training and Research Hospital, Karabaglar/Izmir, Turkey.
Asian Pac J Cancer Prev. 2011;12(4):1077-81.
For detection of Helicobacter pylori, bacterial culture and histopathological examination are invasive in nature, whereas the fast urease test and urea breath test are non-invasive and indirect methods of detection. Stool antibody tests and polymerase chain reaction (PCR) to detect genomic DNA are serological methods, which are preferred to invasive examinations. Our aim was to assess diagnostic specifity and sensitivity of stool antibody tests, with histopathological examination as the golden standard and to compare results with fast urease test findings. Biopsy samples of patients in the study were evaluated as examples of invasive methods, and also stool antibody screening were made (HpSA). When urease and HpSA test results were compared with histopathological results, sensitivity and specificity of urease test were 62.2% and 100%, respectively, and 68.9% and 100% for the HpSA test. General accuracy was 80% and 81%, respectively , positive predictive value 100% with each and negative predictive values 66.1% and 67.2% . The differences were not statistically significant, and the confidence intervals were approximately in the same range. Thus results obtained with biopsy urease and HpSA tests were generally similar to those obtained by histopathological examination. A review of national and international literature showed similar findings.
对于幽门螺杆菌的检测，细菌培养和组织病理学检查本质上是侵入性的，而快速尿素酶试验和尿素呼气试验是非侵入性的间接检测方法。粪便抗体检测和用于检测基因组DNA的聚合酶链反应（PCR）是血清学方法，比侵入性检查更受青睐。我们的目的是以组织病理学检查为金标准，评估粪便抗体检测的诊断特异性和敏感性，并将结果与快速尿素酶试验结果进行比较。研究中患者的活检样本作为侵入性方法的示例进行评估，同时也进行了粪便抗体筛查（HpSA）。当将尿素酶和HpSA检测结果与组织病理学结果进行比较时，尿素酶试验的敏感性和特异性分别为62.2%和100%，HpSA试验的敏感性和特异性分别为68.9%和100%。总体准确率分别为80%和81%，阳性预测值均为100%，阴性预测值分别为66.1%和67.2%。差异无统计学意义，置信区间大致在相同范围内。因此，活检尿素酶试验和HpSA试验获得的结果通常与组织病理学检查获得的结果相似。对国内外文献的综述显示了类似的结果。
