da Silveira Alexsander Augusto, Cardoso Ludimila Paula Vaz, Francisco Roberta Barbosa Lopes, de Araújo Stefani Mariane Martins
Tropical Pathology and Public Health Institute, Federal University of Goiás, Goiânia City/Goiás State, Brazil.
AIDS Res Hum Retroviruses. 2012 Mar;28(3):304-7. doi: 10.1089/aid.2011.0128. Epub 2011 Jul 26.
Antiretroviral naive patients (n=49) were recruited in central western Brazil (Campo Grande City/Mato Grosso do Sul State, located across the Bolivia and Paraguay borders). HIV-1 protease (PR), reverse transcriptase (RT), and env gp41 HR1 fragments were sequenced. Genetic diversity was analyzed by REGA/phylogenetic analyses. Intersubtype recombinants were identified by SimPlot/phylogenetic trees. PR/RT resistance was analyzed by Calibrated Population Resistance/Stanford databases. T-20 resistance in gp41 was assessed by Stanford, Los Alamos, and other sources. Of HIV-1 subtypes 65.3% were B(PR)B(RT), 10.2% were C(PR)C(RT), and 8.2% were F1(PR)F1(RT). Intersubtype recombinants were 16.3%: four B/F1 and four B/C (two were "CRF31_BC-like"). The Pol-RT V75M mutation was detected in two homosexual partners; one patient had the T215S revertant mutation. T-20/gp41 resistance mutations were L44M (n=2) and V38A (n=1). The high percentage of non-B isolates (∼35%) highlights the importance of molecular surveillance studies in settings distant from the origin of the epidemic. Our data help elaborate the molecular epidemiological map of HIV-1 in Brazil.
抗逆转录病毒初治患者（n = 49）招募自巴西中西部（大坎普市/南马托格罗索州，位于玻利维亚和巴拉圭边境）。对HIV-1蛋白酶（PR）、逆转录酶（RT）和env gp41 HR1片段进行测序。通过REGA/系统发育分析来分析基因多样性。通过SimPlot/系统发育树来鉴定亚型间重组体。通过校准群体耐药性/斯坦福数据库来分析PR/RT耐药性。通过斯坦福、洛斯阿拉莫斯及其他来源评估gp41中的T-20耐药性。HIV-1亚型中，65.3%为B(PR)B(RT)，10.2%为C(PR)C(RT)，8.2%为F1(PR)F1(RT)。亚型间重组体占16.3%：四个B/F1和四个B/C（两个为“CRF31_BC样”）。在两名同性恋伴侣中检测到Pol-RT V75M突变；一名患者有T215S回复突变。T-20/gp41耐药性突变有L44M（n = 2）和V38A（n = 1）。非B型分离株的高比例（约35%）凸显了在远离疫情起源地区进行分子监测研究的重要性。我们的数据有助于完善巴西HIV-1的分子流行病学图谱。
