Sanquin Blood Supply, Amsterdam, The Netherlands.
Transfusion. 2012 Jan;52(1):173-80. doi: 10.1111/j.1537-2995.2011.03261.x. Epub 2011 Jul 25.
The platelets (PLTs) in PLT concentrates are counted with hematology analyzers, but varying results among different hematology analyzers are observed, making comparisons very difficult. Due to the absence of red blood cells in PLT concentrates, the International Council for Standardization in Hematology (ICSH) reference method was modified to be used for PLT concentrates and validated in an international comparative study.
Five PLT samples were shipped to eight participating centers of the Biomedical Excellence for Safer Transfusion (BEST) Collaborative and counted on the same day. PLTs were stained with fluorescein isothiocyanate-labeled anti-CD41a in tubes (TruCount, BD Biosciences), measured on a flow cytometer, and analyzed with a uniform template. These samples were also counted on 15 hematology analyzers.
The ICSH method and newly developed BEST method yielded PLT counting results with less than 1% difference (not significant). The intercenter coefficient of variation (CV) of the BEST method was on average 6.3% versus 7.6% on average for hematology analyzers. The CV of individual hematology analyzers was on average 0.9%, which was considerably lower than for the flow cytometers with a mean of 3.7%.
The BEST flow cytometric method has a smaller intercenter CV and a smaller center-to-center deviation from the group mean compared to hematology analyzers. Conversely, individual hematology analyzers are more precise than the flow cytometric method. Thus, the flow cytometric method provides a calibration tool to allow comparisons between centers, but there is no need to replace routine counting with hematology analyzers.
血小板（PLT）在 PLT 浓缩物中通过血液分析仪进行计数，但不同血液分析仪之间存在差异，使得比较变得非常困难。由于 PLT 浓缩物中没有红细胞，国际血液学标准化委员会（ICSH）参考方法经过修改后用于 PLT 浓缩物，并在国际比较研究中得到验证。
将五个 PLT 样本运送到 Biomedical Excellence for Safer Transfusion（BEST）协作的八个参与中心，并在同一天进行计数。PLT 在管中用荧光素异硫氰酸酯标记的抗 CD41a 染色（BD Biosciences 的 TruCount），在流式细胞仪上测量，并使用统一模板进行分析。这些样本还在 15 个血液分析仪上进行了计数。
ICSH 方法和新开发的 BEST 方法得出的 PLT 计数结果差异小于 1%（无显著性）。BEST 方法的中心间变异系数（CV）平均为 6.3%，而血液分析仪的平均 CV 为 7.6%。个别血液分析仪的 CV 平均为 0.9%，明显低于流式细胞仪的 3.7%。
与血液分析仪相比，BEST 流式细胞术方法的中心间 CV 较小，与组平均值的中心间偏差较小。相反，个别血液分析仪比流式细胞术方法更精确。因此，流式细胞术方法提供了一个校准工具，允许中心之间进行比较，但没有必要用血液分析仪代替常规计数。
