Department of Morphology, Applied Microbiology Laboratory, Federal University of Sergipe-FUS, São Cristóvão-SE, Brazil.
Mycoses. 2012 Jan;55(1):64-72. doi: 10.1111/j.1439-0507.2011.02043.x. Epub 2011 Jul 27.
Twenty-eight Candida albicans strains obtained from women with vaginal candidiasis were tested for phospholipase and proteinase production and clustered by multilocus enzyme electrophoresis (MLEE). The proteolytic and phospholipidic activity were considered moderate (0.56 ± 0.12 mm and 0.53 ± 0.09 mm, respectively) for all isolates. The isoenzymes malate dehydrogenase (MDH) and sorbitol dehydrogenase (SDH) showed strong intra-specific discriminatory power. The numerical and genetic interpretation of the bands produced by the isoenzymes tested presented similar discriminatory power. The genetic diversity of the isolates was measured by allelic and genic frequency, perceptual index of polymorphic loci (P = 87.5%), average number of alleles per locus, average number of alleles per polymorphic locus, average heterozygosity observed and average heterozygosity expected. We verified that three isoenzymatic loci (Adh, Gdh and Sdh-2) were not in Hardy-Weinberg equilibrium. A dendrogram constructed based on the genetic distance matrix of Nei showed seven clusters; 57.15% (16) of the isolates were considered highly related or indistinguishable, and 42.85% were considered moderately related or unrelated. We did not find a relationship between the clusters and the exoenzymes production.
28 株从患有阴道念珠菌病的女性中分离得到的白色念珠菌菌株被检测产磷脂酶和蛋白酶的能力，并通过多位点酶电泳（MLEE）聚类。所有分离株的蛋白水解和磷脂活性均被认为是中等水平（分别为 0.56 ± 0.12 mm 和 0.53 ± 0.09 mm）。同工酶苹果酸脱氢酶（MDH）和山梨糖醇脱氢酶（SDH）显示出很强的种内区分能力。同工酶测试产生的带的数值和遗传解释具有相似的区分能力。通过等位基因和基因型频率、多态性位点感知指数（P = 87.5%）、每个位点的平均等位基因数、每个多态性位点的平均等位基因数、观察到的平均杂合度和预期的平均杂合度来衡量分离株的遗传多样性。我们发现三个同工酶基因座（Adh、Gdh 和 Sdh-2）不符合 Hardy-Weinberg 平衡。基于 Nei 遗传距离矩阵构建的聚类树表明有 7 个聚类；57.15%（16 株）的分离株被认为高度相关或无法区分，42.85%被认为中度相关或不相关。我们没有发现聚类与外切酶产生之间的关系。
