Shaha C, Suri A, Talwar G P
Biotechnology Laboratory, National Institute of Immunology, New Delhi, India.
Int J Androl. 1990 Feb;13(1):17-25. doi: 10.1111/j.1365-2605.1990.tb00956.x.
A unique polyvalent antiserum against whole washed human sperm was used previously to identify groups of antigens on spermatozoa. The antiserum, designated as Antiserum I, recognized a 40 kD antigen in human sperm extracts. Antiserum I caused agglutination of human sperm and prevented interaction of mouse sperm and oocytes. This serum also recognized a band of 24 kD in rat testicular cytosol. In the present study this group of 24 kD proteins was used as an antigen preparation to actively immunize female rats. Immunization was carried out with two different adjuvants: nor-muramyl dipeptide and SPLPS (a thyalated derivative of lipopolysaccharide). Both groups of animals showed significant antibody titres as detected by indirect immunofluorescence and by sperm agglutination tests. In both groups over 80% of the animals remained infertile, compared to 13% of the controls. It is concluded that a group of antigens in rat testes recognized by Antiserum I offer promise as candidates for a contraceptive vaccine.
一种独特的针对全洗涤人精子的多价抗血清先前被用于鉴定精子上的抗原组。该抗血清被指定为抗血清I，它能识别出人类精子提取物中的一种40kD抗原。抗血清I会导致人类精子凝集，并阻止小鼠精子与卵母细胞相互作用。这种血清还能识别大鼠睾丸细胞质中的一条24kD条带。在本研究中，将这组24kD蛋白质用作抗原制剂，对雌性大鼠进行主动免疫。使用两种不同的佐剂进行免疫：正常Muramyl二肽和SPLPS（脂多糖的一种硫醇化衍生物）。通过间接免疫荧光和精子凝集试验检测，两组动物均显示出显著的抗体滴度。与13%的对照组相比，两组中超过80%的动物仍保持不育。得出的结论是，抗血清I识别的大鼠睾丸中的一组抗原有望成为避孕疫苗的候选物。
