Que You-Xiong, Lin Jian-Wei, Song Xian-Xian, Xu Li-Ping, Chen Ru-Kai
Key Lab of Sugarcane Genetic Improvement, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.
J Biomed Biotechnol. 2011;2011:160934. doi: 10.1155/2011/160934. Epub 2011 Jul 14.
Differential gene expression in sugarcane during sugarcane-Ustilago scitaminea interaction was conducted in a smut-resistant genotype. Using cDNA-AFLP along with silver staining, a total of 136 transcript-derived fragments (TDFs) were found to be differentially expressed in response to challenge by U. scitaminea. Forty TDFs, 34 newly induced plus six with obvious upregulated expression after infection, were sequenced and validated by RT-PCR analysis. These results demonstrated that the expression of 37 out of these TDFs in RT-PCR analysis was consistent with that in cDNA-AFLP analysis. Based on BlastX in NCBI, 28 TDFs were assumed to function in sugarcane under U. scitaminea stress. Analysis of expression profile of three TDFs revealed that they responded differently after infection with U. scitaminea, and the transcription was significantly enhanced. The response of two TDFs, SUC06 and SUC09, occurred before that of SUC10. This study enriches our knowledge of the molecular basis for sugarcane response to U. scitaminea infection.
在一个抗黑穗病基因型的甘蔗中，研究了甘蔗与甘蔗黑粉菌相互作用期间的差异基因表达。利用cDNA-AFLP结合银染技术，发现共有136个转录衍生片段（TDFs）在受到甘蔗黑粉菌攻击时差异表达。对40个TDFs进行了测序，其中34个是新诱导的，6个在感染后表达明显上调，并通过RT-PCR分析进行了验证。这些结果表明，在RT-PCR分析中，这些TDFs中有37个的表达与cDNA-AFLP分析中的表达一致。基于NCBI中的BlastX，推测28个TDFs在甘蔗黑粉菌胁迫下的甘蔗中发挥作用。对三个TDFs的表达谱分析表明，它们在感染甘蔗黑粉菌后反应不同，转录显著增强。两个TDFs，SUC06和SUC09的反应比SUC10早。本研究丰富了我们对甘蔗响应甘蔗黑粉菌感染的分子基础的认识。
