Neuroscience Program, Federal University of Rio de Janeiro State (UNIRIO), Rio de Janeiro, Brazil.
Biofactors. 2011 Jul-Aug;37(4):315-22. doi: 10.1002/biof.171. Epub 2011 Jul 26.
Cerebrovascular disease studies have shown similarity between humans and spontaneously hypertensive rats stroke-prone rats in the development of spontaneous stroke and transitory ischemic attacks (TIA). In addition, nitric oxide (NO) suppression by L-arginine methyl ester (L-NAME) can precipitate several vascular diseases including TIA and strokes. On the other hand, alpha-tocopherol (AT) has been associated with beneficial effects on vascular disorders. Four groups were tested to evaluate AT effects on NO inhibition: AT, control (C), AT + L-NAME, and L-NAME. During 4 weeks, all groups had their physiologic parameters evaluated and were submitted to neurological tests. After the sacrifice of the animals, total L-lactate dehydrogenase, fibrinogen levels, and platelet counts were measured. Our results demonstrated improvement in memory function and sensory-motor function of the rats treated with AT. The AT treatment also demonstrated a significant difference on the injury identifier, fibrinogen levels, and platelet count between the treated groups and the L-NAME group. In conclusion, AT reversed damaging L-NAME neurological effects and could be considered as a possible protective agent in neurological diseases.
脑血管疾病研究表明，在自发性中风和短暂性脑缺血发作（TIA）的发展方面，人类与自发性高血压大鼠卒中易发性大鼠具有相似性。此外，L-精氨酸甲酯（L-NAME）抑制一氧化氮（NO）可以引发包括 TIA 和中风在内的多种血管疾病。另一方面，α-生育酚（AT）与血管疾病的有益作用有关。为了评估 AT 对 NO 抑制的作用，我们测试了四组：AT、对照组（C）、AT+L-NAME 和 L-NAME。在 4 周的时间里，所有组都评估了它们的生理参数，并进行了神经学测试。在动物被处死之后，测量了总 L-乳酸脱氢酶、纤维蛋白原水平和血小板计数。我们的结果表明，AT 治疗改善了大鼠的记忆功能和感觉运动功能。AT 治疗还显示，在损伤标志物、纤维蛋白原水平和血小板计数方面，治疗组与 L-NAME 组之间存在显著差异。总之，AT 逆转了 L-NAME 神经损伤的有害作用，可被视为神经疾病的一种潜在保护剂。
