State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
J Environ Sci (China). 2011;23(4):671-5. doi: 10.1016/s1001-0742(10)60461-5.
It is generally known that there are many endocrine disrupting compounds (EDCs) in the effluents from wastewater treatment plants (WWTPs). Most research has focused on the occurrence of estrogenic or androgenic activities, while ignoring that there are environmental chemicals disrupting thyroid system, which is essential for growth and development in both humans and animals. In the present work, a two-hybrid yeast assay was conducted to evaluate the removal efficiencies of agonistic or antagonistic thyroid receptor (TR) mediated effects in different treatment processes of three WWTPs located in Beijing. We found no TR agonistic, but TR antagonistic activities in all processes from the WWTPs. The TR antagonistic activities in organic extracts of water samples were then calibrated regarding to a known TR-inhibitor, amiodarone hydrochloride (AH). The observed concentration of TR disrupting substances ranged from 2.35 x 10(-8) to 6.19 x 10(-7) mol/L AH in Gaobeidian WWTP, 3.76 x 10(-8) to 8.75 x 10(-8) mol/L AH in Lugouqiao WWTP, and 4.80 x 10(-9) to 2.55 x 10(-8) mol/L AH in Beixiaohe WWTP. Of the three WWTPs, the removal rates were 92.7%, 42.2%, and 23.1% respectively. Industrial sewage may contain more TR disrupting substances compared with domestic sewage. The recipient waters were found to contain considerable concentrations of TR disrupting substances that may cause adverse effects on the exposed organisms.
人们普遍知道，污水处理厂（WWTP）的废水中存在许多内分泌干扰化合物（EDCs）。大多数研究都集中在雌激素或雄激素活性的发生上，而忽略了环境化学物质对甲状腺系统的干扰，甲状腺系统对人类和动物的生长发育至关重要。在本研究中，我们采用双杂交酵母试验评估了 3 个位于北京的 WWTP 不同处理工艺对甲状腺受体（TR）介导的激动或拮抗作用的去除效率。我们发现所有 WWTP 的工艺过程均不存在 TR 激动作用，但存在 TR 拮抗作用。然后，我们根据已知的 TR 抑制剂盐酸胺碘酮（AH）对水样中有机提取物的 TR 拮抗活性进行了标定。所观察到的 TR 干扰物质的浓度范围为 2.35×10（-8）至 6.19×10（-7）mol/L AH 于高碑店 WWTP，3.76×10（-8）至 8.75×10（-8）mol/L AH 于卢沟桥 WWTP，4.80×10（-9）至 2.55×10（-8）mol/L AH 于北小河 WWTP。在这 3 个 WWTP 中，去除率分别为 92.7%、42.2%和 23.1%。与生活污水相比，工业污水可能含有更多的 TR 干扰物质。结果表明，受纳水体中含有相当浓度的 TR 干扰物质，可能对暴露的生物产生不利影响。
