College of Life Science, Chongqing Normal University, Chongqing, China.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2011 Nov;28(11):1516-23. doi: 10.1080/19440049.2011.589037. Epub 2011 Jul 27.
Two rapid and sensitive enzyme-linked immunosorbent assays (ELISA) and an immunochromatographic assay (ICA) for the detection of chlortetracycline (CTC) residues in edible animal tissues were developed based on a monoclonal antibody (MAb) produced by using the chlortetracycline-bovine serum albumin (CTC-BSA) conjugate as the immunogen. A total of 50% inhibiting concentration (IC(50)) of the modified ELISA was 0.66 ng ml(-1) and the recoveries from spiked chicken muscle and liver were 78.8-92.2% and 80.3-90.2%, respectively. The corresponding coefficient variations (CVs) were 3.2-9.5% and 6.5-10.2%. The detection limit was 0.06 ng g(-1) in chicken muscle and 0.07 ng g(-1) in liver. However, the detection limit of ICA was 0.12 ng ml(-1), and the recoveries in negative samples spiked at concentrations of 10, 50 and 100 ng g(-1) ranged from 79.0% to 88.6% for muscle samples and from 75.2% to 87.0% for liver samples. The cut-off values for the test lines were 80 ng g(-1) and the analysis can be completed within 5-10 min. Comparisons with an HPLC method were performed by testing 200 swine muscle samples and chicken muscle samples from local markets, and an agreement rate of 99.5% was obtained between the three methods.
基于氯四环素-牛血清白蛋白（CTC-BSA）缀合物作为免疫原，制备了两种快速灵敏的酶联免疫吸附测定法（ELISA）和一种免疫层析测定法（ICA），用于检测食用动物组织中的氯四环素（CTC）残留。改良 ELISA 的 50%抑制浓度（IC（50））为 0.66ng/ml，从鸡肉和肝脏中添加的回收率分别为 78.8-92.2%和 80.3-90.2%。相应的变异系数（CV）分别为 3.2-9.5%和 6.5-10.2%。鸡肉中的检测限为 0.06ng/g，肝脏中的检测限为 0.07ng/g。然而，ICA 的检测限为 0.12ng/ml，在浓度为 10、50 和 100ng/g 时，阴性样品的添加回收率在肌肉样品中范围为 79.0%-88.6%，在肝脏样品中范围为 75.2%-87.0%。测试线的截止值为 80ng/g，分析可在 5-10 分钟内完成。通过检测 200 份猪肌肉样本和当地市场的鸡肉样本，与 HPLC 方法进行了比较，三种方法之间的符合率为 99.5%。
