Jia Lei, Xu Zhitao, Chen Rujie, Chen Xiangzhen, Xu Jun
College of Chemistry and Chemical Engineering, Henan Polytechnic University, Jiaozuo 454000, China.
Nanomaterials (Basel). 2021 Dec 31;12(1):128. doi: 10.3390/nano12010128.
Tetracycline (TC) and oxytetracycline (OTC) are the most widely used broad-spectrum antimicrobial agents in tetracycline drugs, and their structures and properties are very similar, so it is a great challenge to distinguish and detect these two antibiotics with a single probe at the same time. Herein, a dual-channel fluorescence probe (SiCDs@mMIPs-cit-Eu) was developed by integrating two independent reaction sites with SiCDs-doped mesoporous silica molecular imprinting group and europium complex group into a nanomaterial. The synergistic influence of inner filter effect and "antenna effect" can be guaranteed to solve the distinction between TC and OTC. Moreover, this novel strategy can also sequentially detect TC and OTC in buffer solution and real samples with high sensitivity and selectivity. This method revealed good responses to TC and OTC ranging from 0 to 5.5 μM with a detection limit of 5 and 16 nM, respectively. Combined with the smartphone color-scanning application, the portable and cheap paper-based sensor was designed to realize the multi-color visual on-site detection of TC and OTC. In addition, the logic gate device was constructed according to the fluorescence color change of the probe for TC and OTC, which provided the application possibility for the intelligent detection of the probe.
四环素（TC）和土霉素（OTC）是四环素类药物中应用最广泛的广谱抗菌剂，它们的结构和性质非常相似，因此用单一探针同时区分和检测这两种抗生素是一项巨大的挑战。在此，通过将两个独立的反应位点与掺杂SiCDs的介孔二氧化硅分子印迹基团和铕配合物基团整合到一种纳米材料中，开发了一种双通道荧光探针（SiCDs@mMIPs-cit-Eu）。可以保证内滤光效应和“天线效应”的协同影响，以解决TC和OTC之间的区分问题。此外，这种新策略还可以在缓冲溶液和实际样品中以高灵敏度和选择性依次检测TC和OTC。该方法对0至5.5μM的TC和OTC显示出良好的响应，检测限分别为5和16 nM。结合智能手机颜色扫描应用，设计了便携式且廉价的纸质传感器，以实现TC和OTC的多色可视化现场检测。此外，根据探针针对TC和OTC的荧光颜色变化构建了逻辑门装置，这为探针的智能检测提供了应用可能性。
