Division of Environmental Medicine, Norwegian Institute of Public Health, P.O. Box 4404 Nydalen, Oslo, Norway.
Nanotoxicology. 2012 Nov;6(7):700-12. doi: 10.3109/17435390.2011.604441. Epub 2011 Jul 28.
Amorphous silica nanoparticles (SiNPs, 30 and 50 nm) and rhodamine-coated SiNPs (50 nm) were examined for their ability to induce pro-inflammatory responses and cytotoxicity in BEAS-2B cells under different experimental conditions. The SiNPs formed micrometre-sized agglomerates in the absence of bovine serum albumin (BSA) in the culture medium, whereas with BSA (0.1%) they were much less agglomerated. All the SiNPs induced IL-6 and IL-8 responses, as measured by ELISA and real-time PCR. The responses were more marked without BSA and higher for the rhodamine SiNPs than the plain ones. Rhodamine SiNPs were not taken up by cells during a 3-h exposure, even though cytokine mRNAs were up-regulated. In conclusion, agglomerated SiNPs induced more potent cytokine responses than the non-agglomerated ones; either due to the agglomeration state per se or more conceivably to a change in surface reactivity against cellular targets due to BSA. Furthermore, cytokine expression was up-regulated independently of SiNP uptake.
无定形二氧化硅纳米颗粒（SiNPs，30nm 和 50nm）和罗丹明包覆的 SiNPs（50nm）在不同的实验条件下，被检测其在 BEAS-2B 细胞中诱导促炎反应和细胞毒性的能力。在培养基中不存在牛血清白蛋白（BSA）的情况下，SiNPs 形成了微米大小的团聚体，而加入 0.1%的 BSA 后，它们的团聚体则少得多。所有 SiNPs 均通过 ELISA 和实时 PCR 测量诱导了 IL-6 和 IL-8 的反应。在没有 BSA 的情况下，反应更为明显，而罗丹明 SiNPs 的反应比普通 SiNPs 更为明显。罗丹明 SiNPs 在 3 小时的暴露过程中未被细胞摄取，尽管细胞因子的 mRNA 被上调。总之，与非团聚的 SiNPs 相比，团聚的 SiNPs 诱导了更强的细胞因子反应；这可能是由于团聚状态本身，或者更可能是由于 BSA 导致表面反应性针对细胞靶标发生了变化。此外，细胞因子的表达上调与 SiNP 的摄取无关。
