Department of Occupational Health Engineering, School of Public Health, Iran University of Medical Sciences, Tehran, Iran.
Pharmaceutical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Sci Rep. 2024 Jun 24;14(1):14484. doi: 10.1038/s41598-024-65333-5.
Among the myriad of nanoparticles, silica nanoparticles (SiONPs) have gained significant attention since they are extensively produced and used across several kinds of industries. Because of its widespread usage, there has been increasing concern about the potential health effects. This study aims to evaluate the effects of SiONPs on Interleukin-6 (IL-6) gene expression in human lung epithelial cell lines (A549). In this study, A549 cells were exposed to SiONPs at concentrations of 0, 1, 10, 50, 100, and 200 µg/mL for 24 and 48 h. The IL-6 gene expression was assessed using Real-Time RT-PCR. Additionally, the impact of SiONPs on the viability of A549 cells was determined by MTT assay. Statistical analysis was performed using GraphPad Prism software 8.0. MTT assay results indicated a concentration-dependent impact on cell survival. After 24 h, survival decreased from 80 to 68% (1-100 µg/mL), rising to 77% at higher concentrations. After 48 h, survival dropped from 97 to 80%, decreasing to 90% at higher concentrations. RT-PCR showed a dose-response relationship in cellular toxicity up to 10 µg/mL. At higher concentrations, there was increased IL-6 gene expression, mitigating SiONP-induced cytotoxic effects. The study shows that the viability and proliferation of A549 cells are impacted by different SiONPs concentrations. There may be a potential correlation between IL-6 gene expression reduction and a mechanism linked to cellular toxicity. However, at higher concentrations, an unknown mechanism increases IL-6 gene expression, reducing SiONPs' cytotoxic effects. These effects are concentration-dependent and not influenced by exposure times. Further investigation is recommended to determine this mechanism's nature and implications, particularly in cancer research.
在众多纳米粒子中，硅纳米粒子（SiONPs）由于在许多行业中广泛生产和使用而受到了极大的关注。由于其广泛的应用，人们越来越关注其潜在的健康影响。本研究旨在评估 SiONPs 对人肺上皮细胞系（A549）中白细胞介素 6（IL-6）基因表达的影响。在本研究中，将 A549 细胞暴露于 0、1、10、50、100 和 200μg/ml 的 SiONPs 浓度下 24 和 48 小时。使用实时 RT-PCR 评估 IL-6 基因表达。此外，通过 MTT 测定法评估 SiONPs 对 A549 细胞活力的影响。使用 GraphPad Prism 软件 8.0 进行统计分析。MTT 测定结果表明，细胞存活率存在浓度依赖性影响。24 小时后，存活率从 80%降至 68%（1-100μg/ml），在较高浓度下上升至 77%。48 小时后，存活率从 97%降至 80%，在较高浓度下降至 90%。RT-PCR 显示细胞毒性呈剂量反应关系，最高可达 10μg/ml。在较高浓度下，IL-6 基因表达增加，减轻了 SiONP 诱导的细胞毒性作用。研究表明，不同浓度的 SiONPs 会影响 A549 细胞的活力和增殖。在细胞毒性与 IL-6 基因表达降低之间可能存在潜在相关性，但在较高浓度下，一种未知机制会增加 IL-6 基因表达，从而降低 SiONPs 的细胞毒性作用。这些作用是浓度依赖性的，不受暴露时间的影响。建议进一步研究以确定该机制的性质和意义，特别是在癌症研究中。
