Shanks M, Lomonossoff G P
Department of Virus Research, John Innes Institute, Norwich, U.K.
J Gen Virol. 1990 Mar;71 ( Pt 3):735-8. doi: 10.1099/0022-1317-71-3-735.
We have determined the nucleotide sequence of the region of red clover mottle virus (RCMV) bottom component RNA which encodes the RCMV equivalent of the cowpea mosaic virus (CPMV) 24K protease. From the alignment of the deduced amino acid sequence of the RCMV 24K protein with that of the homologous protein from CPMV, we speculate on the relative importance of the various amino acid residues which have been implicated in the catalytic mechanism of comovirus proteases.
我们已经确定了红三叶草斑驳病毒(RCMV)底部组分RNA中编码与豇豆花叶病毒(CPMV)24K蛋白酶等效的RCMV区域的核苷酸序列。通过将RCMV 24K蛋白的推导氨基酸序列与CPMV同源蛋白的序列进行比对,我们推测了在豇豆花叶病毒蛋白酶催化机制中涉及的各种氨基酸残基的相对重要性。