Oxelfelt P, Shanks M, Widmark A K, Lomonossoff G P
Swedish University of Agricultural Sciences, Department of Plant and Forest Protection, Uppsala.
J Gen Virol. 1992 Aug;73 ( Pt 8):2121-4. doi: 10.1099/0022-1317-73-8-2121.
cDNA clones specific for the two genomic RNAs of strain O of the comovirus red clover mottle virus (RCMV) were constructed. Using these clones, in conjunction with clones specific for RNAs of RCMV strain S, local lesion isolates containing reciprocal pseudo-recombinants between strains S and O were identified. Investigation of the biological properties of these pseudo-recombinants showed that the ability of RCMV to infect Chenopodium quinoa is determined by B RNA. The results also suggest that both RNAs are involved in symptom formation in Pisum sativum. Analysis of the strain O clones enabled the sequences at the 3' ends of both genomic RNAs of strain O to be determined. Comparison of these sequences with the corresponding region of the strain S RNAs suggests that the 3' terminal sequences critical for replicase recognition may lie somewhat upstream of the poly(A) tract.
构建了菜豆花叶病毒（RCMV）O株系两种基因组RNA的特异性cDNA克隆。利用这些克隆，结合RCMV S株系RNA的特异性克隆，鉴定出了含有S株系和O株系之间相互假重组体的局部病斑分离物。对这些假重组体生物学特性的研究表明，RCMV感染藜的能力由B RNA决定。结果还表明，两种RNA都参与了豌豆的症状形成。对O株系克隆的分析使得能够确定O株系两种基因组RNA 3'末端的序列。将这些序列与S株系RNA的相应区域进行比较表明，对复制酶识别至关重要的3'末端序列可能位于多聚（A）尾的上游。
