编码假定RNA聚合酶的安第斯马铃薯斑驳病毒B RNA片段的分子克隆与序列分析。
Molecular cloning and sequence analysis of a segment from Andean potato mottle virus B RNA encoding the putative RNA polymerase.
作者信息
Krengiel R, Vincente A C, Weyne M, Shindo N, Brioso P S, Felix D B, Villaroel R, de Oliveira D E, Timmerman B
机构信息
Departamento de Genética, Universidade Federal do Rio de Janeiro, Brazil.
出版信息
J Gen Virol. 1993 Feb;74 ( Pt 2):315-8. doi: 10.1099/0022-1317-74-2-315.
Andean potato mottle virus (APMV), an endemic South American comovirus, has a bipartite genome consisting of two plus-strand RNA molecules (M and B RNA). We have cloned the 3' half of the B RNA and identified the complete sequence of the putative APMV RNA polymerase. The RNA polymerase gene is part of a large polyprotein-encoding open reading frame. The putative, mature RNA polymerase, as deduced by comparison with the related cowpea mosaic virus (CPMV), type member of the comovirus group, is 703 amino acids long and shows a large degree of similarity with CPMV and other RNA polymerases.
安第斯马铃薯斑驳病毒（APMV）是一种南美洲特有的豇豆花叶病毒，其基因组由两个正链RNA分子（M和B RNA）组成。我们克隆了B RNA的3'端一半，并确定了推定的APMV RNA聚合酶的完整序列。RNA聚合酶基因是一个大型多聚蛋白编码开放阅读框的一部分。通过与豇豆花叶病毒组的典型成员、相关的豇豆花叶病毒（CPMV）进行比较推断，推定的成熟RNA聚合酶长703个氨基酸，与CPMV和其他RNA聚合酶有很大程度的相似性。
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