Binnicker Matthew J, Jespersen Deborah J, Rollins Leonard O
Mayo Clinic, 200 First Street SW, SDSC 1-526, Rochester, MN 55905, USA.
Clin Vaccine Immunol. 2011 Sep;18(9):1524-6. doi: 10.1128/CVI.05207-11. Epub 2011 Jul 27.
The goal of this study was to compare the BioPlex 2200 measles, mumps, rubella, and varicella-zoster virus (MMRV) IgG multiplex assays (Bio-Rad Laboratories, Hercules, CA) to routine testing by enzyme immunoassay (EIA). Serum specimens (n = 500) submitted to our reference laboratory for routine MMRV IgG testing by EIA were also tested by the BioPlex assays. Following testing, the BioPlex measles, mumps, rubella, and varicella-zoster virus assays demonstrated agreements of 91.6% (95% confidence interval [CI], 88.8% to 93.7%), 94.2% (95% CI, 91.7% to 95.7%), 94.4% (95% CI, 92.0% to 96.1%), and 91.8% (95% CI, 89.0% to 93.9%), respectively, compared to the results of EIA. Timing studies showed that the BioPlex MMRV assay could provide complete analysis of 100 serum specimens in 1.7 h, compared to 5.5 h by EIA. These data indicate that the BioPlex MMRV IgG assays exhibit comparable performance (93% overall agreement [1,860/2,000 results]; κ = 0.67) to routine testing by EIA. The BioPlex assays allow for the simultaneous detection of all four analytes, thereby eliminating potential aliquot errors and reducing turnaround time.
本研究的目的是将BioPlex 2200麻疹、腮腺炎、风疹和水痘-带状疱疹病毒（MMRV）IgG多重检测法（Bio-Rad实验室，加利福尼亚州赫拉克勒斯）与酶免疫测定法（EIA）的常规检测进行比较。提交至我们参考实验室通过EIA进行常规MMRV IgG检测的血清标本（n = 500）也采用BioPlex检测法进行检测。检测后，与EIA的结果相比，BioPlex麻疹、腮腺炎、风疹和水痘-带状疱疹病毒检测法的一致性分别为91.6%（95%置信区间[CI]，88.8%至93.7%）、94.2%（95%CI，91.7%至95.7%）、94.4%（95%CI，92.0%至96.1%）和91.8%（95%CI，89.0%至93.9%）。时间研究表明，BioPlex MMRV检测法能够在1.7小时内对100份血清标本进行完整分析，而EIA则需要5.5小时。这些数据表明，BioPlex MMRV IgG检测法与EIA常规检测具有相当的性能（总体一致性为93%[1,860/2,000个结果]；κ = 0.67）。BioPlex检测法能够同时检测所有四种分析物，从而消除了潜在的分装误差并缩短了周转时间。
