利用肽核酸探针通过流式细胞术快速鉴定金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌。
Rapid identification of Staphylococcus aureus and methicillin resistance by flow cytometry using a peptide nucleic acid probe.
机构信息
Department of Infectious Disease, Cleveland Clinic, 9500 Euclid Avenue/G-21, Cleveland, OH 44195, USA.
出版信息
J Clin Microbiol. 2011 Sep;49(9):3383-5. doi: 10.1128/JCM.01098-11. Epub 2011 Jul 27.
Abstract
A total of 56 Staphylococcus aureus isolates incubated for 2 h in the presence or absence of oxacillin were analyzed by flow cytometry after labeling with an S. aureus-specific peptide nucleic acid (PNA) probe. Two defined ratios, the paired signal count ratio (PSCR) and the gate signal count ratio (GSCR), differentiated methicillin-resistant S. aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) with sensitivities of 100% each and specificities of 96% and 100%, respectively.
摘要
共对 56 株金黄色葡萄球菌进行分析，这些金黄色葡萄球菌在孵育 2 小时后，用金黄色葡萄球菌特异性肽核酸（PNA）探针进行标记，然后分别在有无苯唑西林的情况下进行流式细胞术检测。两个明确的比值，配对信号计数比（PSCR）和门信号计数比（GSCR），可区分耐甲氧西林金黄色葡萄球菌（MRSA）和甲氧西林敏感金黄色葡萄球菌（MSSA），其敏感性均为 100%，特异性分别为 96%和 100%。
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本文引用的文献
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Rapid differentiation of methicillin-resistant and methicillin-susceptible Staphylococcus aureus by flow cytometry after brief antibiotic exposure.短时间抗生素暴露后流式细胞术快速区分耐甲氧西林和甲氧西林敏感金黄色葡萄球菌。
J Clin Microbiol. 2011 Jun;49(6):2116-20. doi: 10.1128/JCM.02548-10. Epub 2011 Apr 6.
2
Rapid identification of Staphylococcus aureus in blood cultures by a combination of fluorescence in situ hybridization using peptide nucleic acid probes and flow cytometry.通过使用肽核酸探针的荧光原位杂交和流式细胞术相结合的方法快速鉴定血培养中的金黄色葡萄球菌。
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