Furr P M, Taylor-Robinson D
Division of Sexually Transmitted Diseases, Clinical Research Centre, Harrow, Middlesex.
J Med Microbiol. 1990 Mar;31(3):203-6. doi: 10.1099/00222615-31-3-203.
All of five lyophilised cultures of Mycoplasma orale kept for 23 years at room temperature were still viable, as were all but one of 12 lyophilised cultures of six Mycoplasma spp. that had been stored for 18-22 years at 4 degrees C. Similarly, 11 of 13 lyophilised ureaplasma cultures were viable after 8-22 years at 4 degrees C; the titre of organisms in the viable cultures had diminished no more than 100-fold. Seven broth cultures of five different Mycoplasma spp. all proved viable 5-13 years after being frozen and stored at -70 degrees C, although there was up to 10(4)-fold reduction in the titre of organisms in some cultures. Furthermore, 18 (82%) of 22 different Mycoplasma spp., originally lyophilised and then reconstituted and stored at -70 degrees C, were viable after 16 years. Viable organisms were found, with little or no reduction in titre, in all of seven broth cultures of Ureaplasma urealyticum, comprising six serotypes, after storage for 6-10 years at -70 degrees C, but five of 18 broth cultures of other human and animal ureaplasmas stored likewise were not viable after 13-14 years and in a further seven of them the titre of viable organisms had diminished greater than or equal to 10(4)-fold.
保存在室温下23年的5株口腔支原体冻干培养物全部仍具活力，保存在4℃下18 - 22年的6种支原体属的12株冻干培养物中，除1株外全部仍具活力。同样，13株冻干脲原体培养物中的11株在4℃下保存8 - 22年后仍具活力；存活培养物中微生物的滴度降低不超过100倍。5种不同支原体属的7株肉汤培养物在冷冻并保存在-70℃下5 - 13年后全部证明仍具活力，尽管某些培养物中微生物的滴度最多降低了10⁴倍。此外，最初冻干然后复溶并保存在-70℃下的22种不同支原体属中的18株（82%）在16年后仍具活力。解脲脲原体的7株肉汤培养物（包括6个血清型）在-70℃下保存6 - 10年后全部发现有存活微生物，且滴度几乎没有或没有降低，但同样保存的其他人和动物脲原体的18株肉汤培养物中有5株在13 - 14年后不再存活，另外7株中存活微生物的滴度降低了大于或等于10⁴倍。
