Galloway D A, Lukanidin E, Topp W C, Sambrook J
J Gen Virol. 1979 Feb;42(2):339-56. doi: 10.1099/0022-1317-42-2-339.
A set of four isogenic rat cell lines transformed by Ad22+ HEY have been studied. While all of the cell lines synthesize SV40 T antigen, only one expresses adenovirus 2 T antigen: none expresses SV40 V antigen or adenovirus 2 fibre antigen. Three cell lines contain 1 to 2 virus equivalents of SV40 and adenoviral sequences per diploid quantity of rate cell DNA and the fourth line contains five copies of SV40 and 20 copies of the adenovirus genome. At least three of the cell lines contain DNA sequences from the helper adenovirus 2 in addition to sequences from the Ad2+ HEY genome. The patterns of integrated virus sequences are complex suggesting multiple insertions of both adenovirus and SV40 DNA sequences. SV40 can be rescued from three cell lines by fusion with permissive cells.
对一组由Ad22 + HEY转化的四个同基因大鼠细胞系进行了研究。虽然所有细胞系都合成SV40 T抗原，但只有一个表达腺病毒2 T抗原：没有一个表达SV40 V抗原或腺病毒2纤维抗原。三个细胞系在每个二倍体大鼠细胞DNA量中含有1至2个病毒当量的SV40和腺病毒序列，第四个细胞系含有5个SV40拷贝和20个腺病毒基因组拷贝。除了来自Ad2 + HEY基因组的序列外，至少三个细胞系还含有来自辅助腺病毒2的DNA序列。整合病毒序列的模式很复杂，表明腺病毒和SV40 DNA序列都有多次插入。通过与允许细胞融合，可以从三个细胞系中拯救出SV40。
