Galloway D A, Copple C D, McDougall J K
Proc Natl Acad Sci U S A. 1980 Feb;77(2):880-4. doi: 10.1073/pnas.77.2.880.
Herpes simplex virus type 2 (HSV-2) DNA was treated with four restriction endonucleases (EcoRI, HincIII, Bgl II, and Xba I) and eight fragments were purified and labeled with 32P in vitro. The kinetics of renaturation of each of the fragments was measured in the presence of DNA extracted from 333-8-9, a hamster cell line transformed by UV light-inactivated HSV-2 strain 333, and from a series of cloned derivatives and their tumor lines. All of the lines examined contained a partial set of viral sequences present at only a few copies per cell. Passage of the cell lines in tissue culture or in animals resulted in partial loss of viral DNA. Two blocks of sequences were present in most of the lines examined; those mapping at positions 21--33 of the HSV-2 genome were detected in seven of seven cell lines tested and those at positions 60--65 were detected in six of eight. Other sequences from the L component can also be present in the DNA of HSV-2-transformed hamster cells.
用四种限制性内切酶（EcoRI、HincIII、Bgl II和Xba I）处理单纯疱疹病毒2型（HSV - 2）DNA，纯化八个片段并在体外用32P进行标记。在从333 - 8 - 9（一种由紫外线灭活的HSV - 2毒株333转化的仓鼠细胞系）以及一系列克隆衍生物及其肿瘤系提取的DNA存在的情况下，测量每个片段的复性动力学。所有检测的细胞系都含有仅以每个细胞少数拷贝存在的部分病毒序列集。细胞系在组织培养或动物体内传代导致病毒DNA部分丢失。在所检测的大多数细胞系中存在两个序列区；在七个测试的细胞系中检测到位于HSV - 2基因组21 - 33位的序列，在八个中的六个中检测到位于60 - 65位的序列。来自L成分的其他序列也可能存在于HSV - 2转化的仓鼠细胞的DNA中。
