Institute of Materials Science and Engineering, National Taipei University of Technology, Taipei, Taiwan.
Int J Nanomedicine. 2011;6:1405-13. doi: 10.2147/IJN.S20473. Epub 2011 Jul 6.
Our previous study showed that Gusuibu (Drynaria fortunei J. Sm.) can stimulate osteoblast maturation. This study was further designed to evaluate the effects of nanoparticles prepared from the water extract of Gusuibu (WEG) on osteoblast survival and maturation. Primary osteoblasts were exposed to 1, 10, 100, and 1000 μg/mL nanoparticles of WEG (nWEG) for 24, 48, and 72 hours did not affect morphologies, viability, or apoptosis of osteoblasts. In comparison, treatment of osteoblasts with 1000 μg/mL WEG for 72 hours decreased cell viability and induced DNA fragmentation and cell apoptosis. nWEG had better antioxidant bioactivity in protecting osteoblasts from oxidative and nitrosative stress-induced apoptosis than WEG. In addition, nWEG stimulated greater osteoblast maturation than did WEG. Therefore, this study shows that WEG nanoparticles are safer to primary osteoblasts than are normal-sized products, and may promote better bone healing by protecting osteoblasts from apoptotic insults, and by promoting osteogenic maturation.
我们之前的研究表明骨碎补（槲蕨）能促进成骨细胞的成熟。本研究旨在进一步评估骨碎补水提物纳米粒（WEG-np）对成骨细胞存活和成熟的影响。原代成骨细胞分别暴露于浓度为 1、10、100 和 1000μg/ml 的 WEG-np（nWEG）24、48 和 72 小时，未影响成骨细胞的形态、活力或凋亡。相比之下，1000μg/ml WEG 处理 72 小时会降低细胞活力，并诱导 DNA 片段化和细胞凋亡。nWEG 比 WEG 具有更好的抗氧化生物活性，能保护成骨细胞免受氧化和硝化应激诱导的凋亡。此外，nWEG 比 WEG 更能促进成骨细胞的成熟。因此，本研究表明 WEG 纳米粒比普通大小的产品对原代成骨细胞更安全，通过保护成骨细胞免受凋亡损伤，并促进成骨成熟，可能促进更好的骨愈合。
