Loche M P
J Gen Virol. 1979 Feb;42(2):429-33. doi: 10.1099/0022-1317-42-2-429.
In G1-arrested cells infected between 1 and 12 h after having been stimulated by fresh serum to progress to S phase, polyoma virus DNA synthesis proceeded in the first half of S phase, and virus and whole cellular DNA accumulated at about the same time. However, in cells infected later than 14 h after serum stimulation, virus DNA synthesis was shifted to the next S phase. Thus, a permissive cell attains competence for polyoma virus DNA replication at a precise moment during an S phase initiated by fresh serum, which can efficiently replace the early virus host DNA stimulation function. When cells were incubated in serum that had lost its capacity to stimulate host DNA synthesis by pre-absorption with growing cells, normal yields of polyoma DNA could nevertheless be observed, which shows that extensive replication of host DNA does not seem to be an obligatory condition for virus DNA replication.
在新鲜血清刺激后进入S期的1至12小时内被感染的G1期停滞细胞中，多瘤病毒DNA合成在S期的前半段进行，病毒和整个细胞DNA大约在同一时间积累。然而，在血清刺激后14小时以后被感染的细胞中，病毒DNA合成转移到下一个S期。因此，一个允许性细胞在由新鲜血清引发的S期的精确时刻获得多瘤病毒DNA复制的能力，新鲜血清可以有效地替代早期病毒宿主DNA刺激功能。当细胞在通过与生长细胞预吸收而失去刺激宿主DNA合成能力的血清中培养时，仍可观察到多瘤病毒DNA的正常产量，这表明宿主DNA的广泛复制似乎不是病毒DNA复制的必要条件。
