Burstin S J, Basilico C
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2540-4. doi: 10.1073/pnas.72.7.2540.
A temperature-sensitive mutant of hamster BHK 21/13 cells, tsAF8, which at 39 degrees becomes arrested in the G1 (G0) phase of the cell cycle, is phenotypically altered with respect to temperature sensitivity after transformation with polyoma virus. Polyoma transformation does not produce reversion to a non-temperature-sensitive phenotype but causes increased entry into S and increased rate of cell death at the nonpermissive temperature, compared to untransformed tsAF8 cells. The increased frequency of cells synthesizing DNA is not accompanied by an increased frequency of mitosis, since most of the polyoma-transformed tsAF8 cells that synthesize DNA at the nonpermissive temperature do not divide. At the permissive temperature, polyoma-transformed tsAF8 cells, unlike tsAF8, also lose viability when exposed to other methods of arresting cells in G1. The most likely explanation for this phenomenon is that polyoma virus transformation interferes with the cellular response to this mutation as well as to other conditions that cause cell cycle arrest in G1.
仓鼠BHK 21/13细胞的温度敏感突变体tsAF8，在39摄氏度时会停滞在细胞周期的G1（G0）期，在用多瘤病毒转化后，其温度敏感性的表型发生了改变。与未转化的tsAF8细胞相比，多瘤病毒转化不会产生向非温度敏感表型的回复，但会导致在非允许温度下进入S期的细胞增多以及细胞死亡率增加。合成DNA的细胞频率增加并没有伴随着有丝分裂频率的增加，因为大多数在非允许温度下合成DNA的多瘤病毒转化的tsAF8细胞不进行分裂。在允许温度下，与tsAF8不同，多瘤病毒转化的tsAF8细胞在暴露于其他使细胞停滞在G1期的方法时也会丧失活力。对这一现象最可能的解释是，多瘤病毒转化干扰了细胞对这种突变以及其他导致细胞周期停滞在G1期的条件的反应。
