Dou Chang, Xu Qing, Song Ping, Jiang Ling, Li Shuang
College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China.
Wei Sheng Wu Xue Bao. 2011 Apr;51(4):468-73.
As one of the most abandon and cheapest recyclable bio-resources, cellulosic biomass appear to be the promising new energy if the two major components, glucose and xylose, are efficiently used.
We compared the metabolism of Rhizopus oryzae growing on glucose or xylose. We measured biomass accumulation, intercellular metabolite contents, and organic acids production.
When cultured on xylose, the rate of bio-transformation reached 35.2% compared to 24.3% in the glucose. Intercellular contents accumulated much more by using xylose as sole carbon than using glucose. However, the quantity of organic acid was less with xylose than with glucose. In addition, during the coexistence of both substrates, Rhizopus oryzae utilizes glucose first, and xylose later when the glucose is almost exhausted.
With our data analysis, both the NADH/NAD + and ATP quantity are differed between the utilization of two carbon resources. In xylose, biological macromolecule and biomass accumulation are in advantage, while in glucose, the inorganic acid production is enhanced.
作为最被废弃且最便宜的可回收生物资源之一，如果纤维素生物质的两种主要成分葡萄糖和木糖能得到有效利用，那么它似乎是一种很有前景的新能源。
我们比较了米根霉在葡萄糖或木糖上生长时的代谢情况。我们测量了生物量积累、细胞内代谢物含量和有机酸产量。
当在木糖上培养时，生物转化速率达到35.2%，而在葡萄糖上为24.3%。以木糖作为唯一碳源时细胞内物质积累比使用葡萄糖时多得多。然而，木糖产生的有机酸量比葡萄糖少。此外，在两种底物共存时，米根霉先利用葡萄糖，当葡萄糖几乎耗尽时再利用木糖。
通过我们的数据分析，利用两种碳源时NADH/NAD +和ATP的量均有差异。在木糖中，生物大分子和生物量积累具有优势，而在葡萄糖中，无机酸产量增加。
