Wright B E, Longacre A, Reimers J
University of Montana, Division of Biological Sciences, Missouia 59812, USA.
J Theor Biol. 1996 Oct 7;182(3):453-7. doi: 10.1006/jtbi.1996.0186.
A flux analysis of glucose metabolism in Rhizopus oryzae was achieved using [14C]-labeled glucose and acetate. The rates of glucose utilization and end product production were estimated, and metabolite pool sizes and specific radioactivities were determined. These data were analysed using a specific radioactivity curve-matching program called TFLUX. The analysis is consistent with the existence of separate mitochondrial and cytosolic pools of pyruvate, malate and fumarate.
利用[14C]标记的葡萄糖和乙酸盐对米根霉中的葡萄糖代谢进行了通量分析。估算了葡萄糖利用速率和终产物生成速率，并测定了代谢物池大小和比放射性。使用一个名为TFLUX的比放射性曲线匹配程序对这些数据进行了分析。该分析结果与丙酮酸、苹果酸和富马酸在线粒体和胞质溶胶中存在独立的代谢池这一情况相符。
