Biomedical Sciences Department, Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire, SP4 0JQ, UK.
Microb Pathog. 2011 Dec;51(6):471-5. doi: 10.1016/j.micpath.2011.07.002. Epub 2011 Jul 20.
A variety of studies have implicated neutrophils and the rapid induction of cytokine in the host response in melioidosis. Here a BALB/c mouse model of infection with aerosolised Burkholderia pseudomallei K96243 has been used to understand the immune response to infection in this model and verify other infection models that show rapid growth of bacteria, colonisation of tissues and periphery, induction of cytokines and influx of neutrophils. Uniquely, this study has also determined the association of B. pseudomallei to host cells in vivo using flow cytometric techniques. Neutrophils were found to be the predominant cell-type exhibiting B. pseudomallei antigens during infection and it is likely that bacteria have been internalised. This data confirms that neutrophils are likely to play an important and active role in fighting infection with B. pseudomallei.
多种研究表明中性粒细胞和细胞因子的迅速诱导与类鼻疽病宿主反应有关。在这里，我们使用气溶胶化伯克霍尔德菌假单胞菌 K96243 感染的 BALB/c 小鼠模型来了解该模型中的感染免疫反应，并验证其他感染模型，这些模型显示出细菌的快速生长、组织和外周的定植、细胞因子的诱导和中性粒细胞的涌入。独特的是，这项研究还使用流式细胞术技术确定了体内假单胞菌与宿主细胞的关联。在感染过程中发现中性粒细胞是表达假单胞菌抗原的主要细胞类型，细菌很可能已经被内化。这些数据证实，中性粒细胞很可能在对抗假单胞菌感染方面发挥重要而积极的作用。
