Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, UFMG 31270-901, Belo Horizonte, MG, Brazil.
Phytochemistry. 2011 Nov;72(16):1947-54. doi: 10.1016/j.phytochem.2011.06.010. Epub 2011 Jul 26.
Cysteine proteinases from the Caricaceae belong to the C1 family of the CA clan and display papain-like structured, the archetype enzyme for this group of proteins. Carica candamarcensis, also named Vasconcellea cundinamarcensis, a member of Caricaceae family common to many areas in South America, contains cysteine proteinases with proteolytic activity five to eight-fold higher than those from latex of Carica papaya. The cysteine protease CMS2MS2 from C. candamarcensis latex has been shown to enhance proliferation of L929 fibroblast and to activate the extracellular signal-regulated protein kinase (ERK). In this study, the cDNA cloning, expression and evaluation of biological activity of a CMS2MS2-like protein from C. candamarcensis is reported. The 650 bp fragment was cloned in bacteria and the DNA sequence confirmed a cysteine-proteinase similar to CMS2MS2. The recombinant protein is 30 kDa, induces a mitogenic response, and enhances ERK1/2 phosphorylation, like the non-recombinant enzyme, but lacks either amidase or caseinolytic activity. The mitogenic activity of this protein and its lack of proteolytic activity underscore a potential for use in wound healing treatment.
Caricaceae 中的半胱氨酸蛋白酶属于 CA 家族的 C1 家族，具有类似于木瓜蛋白酶的结构，是该组蛋白质的典型酶。Carica candamarcensis，也称为 Vasconcellea cundinamarcensis，是南美洲许多地区常见的 Caricaceae 科的一员，其半胱氨酸蛋白酶具有比木瓜乳汁中更高的五到八倍的蛋白水解活性。来自 C. candamarcensis 乳汁的半胱氨酸蛋白酶 CMS2MS2 已被证明可增强 L929 成纤维细胞的增殖，并激活细胞外信号调节蛋白激酶（ERK）。在这项研究中，报道了来自 C. candamarcensis 的 CMS2MS2 样蛋白的 cDNA 克隆、表达和生物活性评价。在细菌中克隆了 650 bp 片段，并且 DNA 序列证实了一种类似于 CMS2MS2 的半胱氨酸蛋白酶。重组蛋白为 30 kDa，诱导有丝分裂反应，并增强 ERK1/2 磷酸化，与非重组酶相似，但缺乏酰胺酶或酪蛋白水解活性。这种蛋白质的有丝分裂活性及其缺乏蛋白水解活性突出了其在伤口愈合治疗中的潜在用途。
