Gomes Marco Túlio R, Mello Vanessa J, Rodrigues Kelly C, Bemquerer Marcelo P, Lopes Miriam T P, Faça Vitor M, Salas Carlos E
Departamentos de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antonio Carlos 6627, Belo Horizonte, Minas Gerais 31270-901, Brazil.
Planta Med. 2005 Mar;71(3):244-8. doi: 10.1055/s-2005-837824.
In a prior study we showed evidence that latex from Carica candamarcensis contains a protein fraction that stimulates mammalian cell proliferation. In this report we describe the isolation of two proteinases responsible for this effect. Both proteinases (P1, P2) display a relative mass of 23 kDa and following chromatographic purification stimulate proliferation of fibroblastic and epithelial cells. P2 added to L929 fibroblasts at 2.5 nM enhances proliferation by 60 %. We further demonstrate that its cellular effect is linked to an increase in activity of Erk2, a component of the MAP kinase pathway. To our knowledge, this is the first known plant proteinase to exert a proliferative effect in mammalian cells. This novel mitogenic property attributed to a purified cysteine proteinase may explain some of the therapeutic actions attributed to these enzymes.
在之前的一项研究中，我们发现来自Candamarcensis番木瓜的乳胶含有一种能刺激哺乳动物细胞增殖的蛋白质组分。在本报告中，我们描述了两种造成这种效应的蛋白酶的分离过程。这两种蛋白酶（P1、P2）的相对分子质量均为23 kDa，经色谱纯化后可刺激成纤维细胞和上皮细胞的增殖。以2.5 nM的浓度添加到L929成纤维细胞中的P2可使细胞增殖提高60%。我们进一步证明，其细胞效应与MAP激酶途径的组成部分Erk2的活性增加有关。据我们所知，这是已知的第一种在哺乳动物细胞中发挥增殖作用的植物蛋白酶。这种归因于纯化半胱氨酸蛋白酶的新的促有丝分裂特性可能解释了这些酶的一些治疗作用。
