Silva C A, Gomes M T, Ferreira R S, Rodrigues K C, Val C G, Lopes M T, Mello V J, Salas C E
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, UFMG, Belo Horizonte, Brasil.
Planta Med. 2003 Oct;69(10):926-32. doi: 10.1055/s-2003-45102.
Latex from Caricaceae contains a number of proteins believed to be part of a defense mechanism that protects these plants from wounding. Prior evidence suggests that some components in Carica papaya improve healing of ulcerous wounds in mammals. This study shows the chromatographic isolation of a protein fraction from C. candamarcensis that stimulates cell proliferation of mammalian cells by measuring MTT reduction and thymidine incorporation. The effect appears to be cell specific as L929, MDA-MB231 and BHK-21 cells are stimulated while no effect is seen on CHO cells. The maximal stimulatory effect reaches 2.2-fold 72 h after addition of the active fraction to L929, 1.8-fold in MDA-MB231 cells and 1.6-fold in BHK cells. Proteolytic inactivation of the active fraction suggests that a protein is responsible for the proliferative activity and its size is estimated between 10 and 25 kDa. A potential candidate for this function is a 23 kDa protein found in the fraction that reacts with human EGF antibody.
番木瓜科植物的乳胶含有多种蛋白质，这些蛋白质被认为是保护这些植物免受伤害的防御机制的一部分。先前的证据表明，番木瓜中的某些成分可促进哺乳动物溃疡伤口的愈合。本研究通过测量MTT还原和胸腺嘧啶核苷掺入，展示了从卡丹马克番木瓜中色谱分离出一种蛋白质组分，该组分可刺激哺乳动物细胞的增殖。这种作用似乎具有细胞特异性，因为L929、MDA-MB231和BHK-21细胞受到刺激，而对CHO细胞则无影响。在向L929细胞添加活性组分72小时后，最大刺激作用达到2.2倍，在MDA-MB231细胞中为1.8倍，在BHK细胞中为1.6倍。活性组分的蛋白水解失活表明，一种蛋白质负责增殖活性，其大小估计在10至25 kDa之间。该功能的一个潜在候选者是在该组分中发现的一种23 kDa蛋白质，它与人表皮生长因子抗体发生反应。
