Institute of Medical Psychology, School of Medicine, Shandong University, Jinan, PR China.
Ann Anat. 2011 Oct 20;193(5):418-24. doi: 10.1016/j.aanat.2011.06.001. Epub 2011 Jul 8.
This preliminary study aims to explore how adrenergic agents modulate stress response and affect stress-induced behavioral and brain changes in rodents. A total of 40 adult male Wistar rats were subjected to chronic unpredictable mild stress (CUMS) and randomly divided into five groups. At 30 min before daily stress exposure, the rats were intraperitoneally injected with phentolamine (5mg/kg), noradrenalin (1.0mg/kg), propranolol (10mg/kg), isoproterenol (0.05 mg/kg) or saline, respectively. Another 8 rats served as normal control and received daily saline injection without stress exposure. Open-field behaviors were tested at 1 day after the end of the 21 days of stress exposure. Blood samples were collected for serum corticosterone measurement. Brain sections containing hippocampus were stained with hematoxylin and eosin (H&E) as well as by immunohistochemistry for heat shock protein 70 (hsp70) and nitric oxide synthase type 2 (nos2) analyses. The experimental results demonstrated that repetitive dosing of noradrenalin, phentolamine, and propranolol during chronic stress might region-dependently attenuate stress-induced microstructural and biochemical changes in the hippocampus, although propranolol intensified stress-induced behavioral changes.
本初步研究旨在探讨肾上腺素能激动剂如何调节应激反应，并影响应激引起的啮齿动物行为和大脑变化。将 40 只成年雄性 Wistar 大鼠进行慢性不可预测轻度应激（CUMS）处理，并随机分为五组。在每日应激暴露前 30 分钟，大鼠分别腹膜内注射苯肾上腺素（5mg/kg）、去甲肾上腺素（1.0mg/kg）、普萘洛尔（10mg/kg）、异丙肾上腺素（0.05mg/kg）或生理盐水。另外 8 只大鼠作为正常对照，每日接受生理盐水注射而不进行应激暴露。在应激暴露 21 天后结束的第 1 天进行旷场行为测试。采集血样测量血清皮质酮。用苏木精和伊红（H&E）以及热休克蛋白 70（hsp70）和一氧化氮合酶 2（nos2）免疫组织化学染色对包含海马体的脑切片进行染色。实验结果表明，慢性应激期间重复给予去甲肾上腺素、苯肾上腺素和普萘洛尔可能会区域依赖性地减轻应激引起的海马体微观结构和生化变化，尽管普萘洛尔加剧了应激引起的行为变化。
