DDL Diagnostic Laboratory, Voorburg, The Netherlands.
J Clin Virol. 2011 Oct;52(2):84-7. doi: 10.1016/j.jcv.2011.06.016. Epub 2011 Jul 27.
Large numbers of HPV types infect the human skin and members from the HPV genera alpha, gamma and mu are associated with cutaneous warts.
The aim of this study was to test if the HPV genotypes in swabs of the overlying skin are identical to the types present within these warts.
To this purpose, 25 persons being treated for persistent cutaneous warts were enrolled. Swabs of the overlying skin of the wart were collected from each participant. Additionally, scabs of the wart and deeper portions of the warts were surgically removed. HPV genotyping was performed on all samples using the novel HSL-PCR/MPG assay and the HPV genotyping results were compared.
From the 25 wart biopsies one was HPV negative. 15 were positive for HPV27, 3 for HPV57, 2 for HPV2, 2 for HPV1, 1 for HPV3 and 1wart biopsy was positive for both HPV41 and HPV65. Scabs and swabs of the warts both showed identical typing results as the biopsies in 24 of the 25 cases (sensitivity: 96%).
There was an excellent agreement between HPV types in the swabs of the skin that overlies the warts and the biopsies of these warts validating the use of wart swabs for future studies of wart-associated HPV types. HPV27 was highly prevalent (70%) in the in adults of the investigated population of patients with persistent cutaneous warts.
大量 HPV 类型感染人类皮肤，其中属于 alpha、gamma 和 mu 属的 HPV 与皮肤疣有关。
本研究旨在测试疣表面拭子中的 HPV 基因型是否与这些疣中存在的类型相同。
为此，招募了 25 名正在接受持续性皮肤疣治疗的患者。从每位参与者的疣表面采集拭子。此外，还通过手术切除了疣的痂皮和更深部位。使用新型 HSL-PCR/MPG 检测法对所有样本进行 HPV 基因分型，并比较 HPV 基因分型结果。
在 25 份疣活检样本中，有 1 份 HPV 阴性。15 份为 HPV27 阳性，3 份为 HPV57 阳性，2 份为 HPV2 阳性，2 份为 HPV1 阳性，1 份为 HPV3 阳性，1 份活检为 HPV41 和 HPV65 均阳性。24 例（敏感性：96%）中，疣的痂皮和拭子与疣活检的分型结果完全一致。
在疣表面拭子与这些疣的活检之间，HPV 类型存在极好的一致性，验证了使用疣拭子进行未来与疣相关的 HPV 类型研究的可行性。在研究人群中，持续性皮肤疣患者的成年人中 HPV27 高度流行（70%）。
