Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Potsdam, Germany.
Nat Protoc. 2011 Jul 28;6(8):1209-20. doi: 10.1038/nprot.2011.357.
A detailed protocol for the large-scale synthesis of carbohydrate and dihydrolipoic acid (DHLA)-coated CdSe/ZnS and CdTe/ZnS nanoparticles using continuous flow reactors is described here. Three continuous flow microreaction systems, operating at three different temperatures, are used for the synthesis of mannose-, galactose- or DHLA-functionalized quantum dots (QDs). In the first step of synthesis, the CdSe and CdTe nanoparticles are prepared. The size and spectral properties of the CdSe core of the nanoparticles are controlled by adjustment of the residence time and the temperature. As a second step, the zinc sulfide capping under homogenous conditions is carried out at a substantially lower temperature than is required for nanoparticle growth in batch processes. Finally, the trioctylphosphine/oleic acid ligand is effectively replaced with either carbohydrate PEG-thiol moieties or DHLA at 60 °C. This new protocol allows the synthesis of biologically active fluorescent QDs in 4 d.
这里描述了使用连续流反应器大规模合成碳水化合物和二氢硫辛酸 (DHLA) 涂层 CdSe/ZnS 和 CdTe/ZnS 纳米粒子的详细方案。三个连续流微反应系统在三种不同温度下用于合成甘露糖、半乳糖或 DHLA 功能化量子点 (QD)。在合成的第一步中，制备 CdSe 和 CdTe 纳米粒子。通过调整停留时间和温度来控制纳米粒子的 CdSe 核的尺寸和光谱特性。作为第二步，在比批处理过程中纳米颗粒生长所需的温度低得多的条件下进行同质的硫化锌封端。最后，在 60°C 下，三辛基膦/油酸配体可有效地被碳水化合物 PEG-硫醇部分或 DHLA 取代。该新方案允许在 4 天内合成具有生物活性的荧光 QD。
